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(Plasmid #85808)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85808 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone manufacturer
    Prof. Ikuko Hara-Nishimura
  • Total vector size (bp) 18522
  • Vector type
    Plant Expression, CRISPR
  • Selectable markers
    Hygromycin ; TagRFP in seeds

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    DH5alpha/Mach1 can be used. The cloning efficiency is very low. E. coli with high competency is required.
  • Copy number


  • Gene/Insert name
    human-codon-optimized SpCas9
  • Species
    Streptococcus pyogenes
  • Promoter AtRPS5A
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    human-codon-optimized SpCas9 was closed from pX330-U6-Chimeric_BB-CBh-hSpCas9 (item #42230) that we bought from Addgene on 6th June, 2013 by Prof. Tetsuya Higashiyama
  • Articles Citing this Plasmid

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Although this vector does not has a problem of sgRNA truncation like pKIR1.1, cloning efficiency in vector construction is low. E. coli with high competency is required.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKI1.1R was a gift from Tetsuya Higashiyama (Addgene plasmid # 85808 ; ; RRID:Addgene_85808)
  • For your References section:

    pKAMA-ITACHI vectors for highly efficient CRISPR/Cas9-mediated gene knockout in Arabidopsis thaliana. Tsutsui H, Higashiyama T. Plant Cell Physiol. 2016 Nov 17. pii: pcw191. 10.1093/pcp/pcw191 PubMed 27856772