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pBA904
(Plasmid #122238)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 122238 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pU6-sgRNA EF1Alpha-puro-T2A-BFP (Addgene, #60955)
  • Total vector size (bp) 8914
  • Modifications to backbone
    A modified sgRNA (with capture sequence 1 in stem loop 2 of the constant region/tracr) was inserted using BlpI and XhoI sites.
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin ; BFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    sgRNA with cs1 in stem loop 2 of the constant region
  • gRNA/shRNA sequence
    sgNegCtrl3 - non-targeting
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BlpI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer cagcacaaaaggaaactcacc
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBA904 was a gift from Jonathan Weissman (Addgene plasmid # 122238 ; http://n2t.net/addgene:122238 ; RRID:Addgene_122238)
  • For your References section:

    Combinatorial single-cell CRISPR screens by direct guide RNA capture and targeted sequencing. Replogle JM, Norman TM, Xu A, Hussmann JA, Chen J, Cogan JZ, Meer EJ, Terry JM, Riordan DP, Srinivas N, Fiddes IT, Arthur JG, Alvarado LJ, Pfeiffer KA, Mikkelsen TS, Weissman JS, Adamson B. Nat Biotechnol. 2020 Aug;38(8):954-961. doi: 10.1038/s41587-020-0470-y. Epub 2020 Mar 30. 10.1038/s41587-020-0470-y PubMed 32231336