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Ultrasensitive Genetically Encoded Indicator for Hydrogen Peroxide Identifies Roles for the Oxidant in Cell Migration and Mitochondrial Function.
Pak VV, Ezerina D, Lyublinskaya OG, Pedre B, Tyurin-Kuzmin PA, Mishina NM, Thauvin M, Young D, Wahni K, Martinez Gache SA, Demidovich AD, Ermakova YG, Maslova YD, Shokhina AG, Eroglu E, Bilan DS, Bogeski I, Michel T, Vriz S, Messens J, Belousov VV
Cell Metab. 2020 Mar 3;31(3):642-653.e6. doi: 10.1016/j.cmet.2020.02.003.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
136464pLifeAct-HyPer7Mammalian expression of F-actin targeted ultrasensitive hydrogen peroxide indicator HyPer7 fused with LifeAct peptide for optical imaging
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136465pCMV-HyPer7-MEMMammalian expression of cytosolic side of plasma membrane targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging
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136466pCS2+HyPer7Mammalian expression of non-targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging
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136467pCS2+HyPer7-NESMammalian expression of cytoplasm targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging
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136468pCS2+HyPer7-NLSMammalian expression of nucleus targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging
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136469pCS2+IMS-HyPer7Mammalian expression of mitochondrial intermembrane space targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging
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136470pCS2+MLS-HyPer7Mammalian expression of mitochondrial matrix targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging
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141071pQE30-HyPer7Bacterial expression of ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging
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Antibodies from Article