The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice.
Yesbolatova A, Saito Y, Kitamoto N, Makino-Itou H, Ajima R, Nakano R, Nakaoka H, Fukui K, Gamo K, Tominari Y, Takeuchi H, Saga Y, Hayashi KI, Kanemaki MT
Nat Commun. 2020 Nov 11;11(1):5701. doi: 10.1038/s41467-020-19532-z. PubMed Article
Yesbolatova A, Saito Y, Kitamoto N, Makino-Itou H, Ajima R, Nakano R, Nakaoka H, Fukui K, Gamo K, Tominari Y, Takeuchi H, Saga Y, Hayashi KI, Kanemaki MT
Nat Commun. 2020 Nov 11;11(1):5701. doi: 10.1038/s41467-020-19532-z. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose | |
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| 140532 | pAY5 (mAID-EGFP-NLS piggyBac) | mAID-EGFP-NLS piggyBac |
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| 140533 | pAY7 (OsTIR1 mAID-EGFP-NLS TOL2) | OsTIR1(WT)-P2A-mAID-EGFP-NLS TOL2 |
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| 140534 | pAY15 (OsTIR1(F74G) mAID-EGFP-NLS) | OsTIR1(F74G)-P2A-mAID-EGFP-NLS TOL2 |
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| 140535 | pAY19 (OsTIR1(F74A) mAID-EGFP-NLS) | OsTIR1(F74A)-P2A-mAID-EGFP-NLS TOL2 |
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| 140536 | pMK381 (AAVS1 CMV-OsTIR1F74G) | AAVS1 CMV-OsTIR1(F74G) |
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| 140537 | pMK233 (AAVS1 CMV-AtAFB2) | AAVS1 CMV-AtAFB2 |
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| 140538 | pMK262 (RAD21-mAC Neo) | RAD21 tagging with mAID-Clover |
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| 140539 | pMK265 (RAD21-mAC Hygro) | RAD21 tagging with mAID-Clover |
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| 140540 | RAD21 C-tag CRISPR | RAD21 tagging CRISPR |
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| 140541 | pMK296 (DHC1-mAID Neo) | DHC1 tagging with mAID |
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| 140542 | pMK297 (DHC1-mAID Hygro) | DHC1 tagging with mAID |
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| 140543 | DHC1-mIAA7 Neo | DHC1 tagging with mIAA7 |
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| 140544 | DHC1-mIAA17 Hygro | DHC1 tagging with mIAA7 |
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| 140545 | DHC1 CRISPR | DHC1 tagging CRISPR |
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| 140645 | CTCF-mAC donor (Neo) | CTCF tagging with mAID-Clover |
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| 140646 | CTCF-mAC donor (Hygro) | CTCF tagging with mAID-clover |
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| 140647 | CTCF-C CRISPR | CTCF tagging CRISPR |
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| 140648 | SMC2-mAC donor (Hygro) | SMC2 tagging with mAID-Clover |
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| 140649 | SMC2 CRISPR | SMC2 tagging CRISPR |
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| 140650 | mAID-BRD4 donor | BRD4 tagging with mAID |
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| 140651 | BRD4-N CRISPR | BRD4 tagging CRISPR |
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| 140652 | pMK321 (TOP2A-mAC (Neo)) | TOP2A tagging with mAID-Clover |
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| 140653 | pMK322 (TOP2A-mAC (Hygro)) | TOP2A tagging with mAID-Clover |
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| 140654 | pMK312 (TOP2A CRISPR) | TOP2A tagging CRISPR |
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| 140655 | pMK198 (GAL-OsTIR1) | GAL-OsTIR1(WT) |
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| 140656 | pMK419 (GAL-OsTIR1(F74G)) | GAL-OsTIR1(F74G) |
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| 140657 | pMK425 (GAL-OsTIR1(F74A)) | GAL-OsTIR1(F74A) |
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| 140658 | pMK427 (OsTIR1(WT) mAID-EGFP-Nluc) | OsTIR1(WT)-P2A-mAID-EGFP-Nluc |
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| 140659 | pMK411 (OsTIR1(F74G) mAID-EGFP-Nluc) | OsTIR1(F74G)-P2A-mAID-EGFP-Nluc |
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| 140729 | pAAV-hSyn-OsTIR1(WT) | OsTIR1(WT)-P2A-mAID-EGFP-NES |
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| 140730 | pAAV-hSyn-OsTIR1(F74G) | OsTIR1(F74G)-P2A-mAID-EGFP-NES |
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| 140731 | pAAV-hSyn-OsTIR1(F74A) | OsTIR1(F74A)-P2A-mAID-EGFP-NES |
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| 158621 | DHC1-AID-Clover donor (Neo) | Donor plasmid for tagging DHC1 with AID-Clover |
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| 158622 | DHC1-AID-Clover donor (Hygro) | Donor plasmid for tagging DHC1 with AID-Clover |
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| 158663 | AAVS1-Tet-OsTIR1(WT)-V5 | Tet-OsTIR1(WT)-V5 donor for integration at the AAVS1 locus |
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| 158664 | AAVS1-Tet-OsTIR1(F74G)-V5 | Tet-OsTIR1(F74G)-V5 donor for integration at the AAVS1 locus |
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| 159992 | pMK395 (AAVS1 CMV-OsTIR1(F74A)) | Expression of OsTIR1(F74A) in human cells |
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