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The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice.
Yesbolatova A, Saito Y, Kitamoto N, Makino-Itou H, Ajima R, Nakano R, Nakaoka H, Fukui K, Gamo K, Tominari Y, Takeuchi H, Saga Y, Hayashi KI, Kanemaki MT.
Nat Commun (2020) 11(1):5701
Article

Plasmids from Article

ID Plasmid Purpose  
140532pAY5 (mAID-EGFP-NLS piggyBac)mAID-EGFP-NLS piggyBac
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140533pAY7 (OsTIR1 mAID-EGFP-NLS TOL2)OsTIR1(WT)-P2A-mAID-EGFP-NLS TOL2
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140534pAY15 (OsTIR1(F74G) mAID-EGFP-NLS)OsTIR1(F74G)-P2A-mAID-EGFP-NLS TOL2
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140535pAY19 (OsTIR1(F74A) mAID-EGFP-NLS)OsTIR1(F74A)-P2A-mAID-EGFP-NLS TOL2
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140536pMK381 (AAVS1 CMV-OsTIR1F74G)AAVS1 CMV-OsTIR1(F74G)
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140537pMK233 (AAVS1 CMV-AtAFB2)AAVS1 CMV-AtAFB2
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140538pMK262 (RAD21-mAC Neo)RAD21 tagging with mAID-Clover
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140539pMK265 (RAD21-mAC Hygro)RAD21 tagging with mAID-Clover
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140540RAD21 C-tag CRISPRRAD21 tagging CRISPR
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140541pMK296 (DHC1-mAID Neo)DHC1 tagging with mAID
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140542pMK297 (DHC1-mAID Hygro)DHC1 tagging with mAID
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140543DHC1-mIAA7 NeoDHC1 tagging with mIAA7
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140544DHC1-mIAA17 HygroDHC1 tagging with mIAA7
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140545DHC1 CRISPRDHC1 tagging CRISPR
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140645CTCF-mAC donor (Neo)CTCF tagging with mAID-Clover
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140646CTCF-mAC donor (Hygro)CTCF tagging with mAID-clover
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140647CTCF-C CRISPRCTCF tagging CRISPR
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140648SMC2-mAC donor (Hygro)SMC2 tagging with mAID-Clover
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140649SMC2 CRISPRSMC2 tagging CRISPR
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140650mAID-BRD4 donorBRD4 tagging with mAID
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140651BRD4-N CRISPRBRD4 tagging CRISPR
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140652pMK321 (TOP2A-mAC (Neo))TOP2A tagging with mAID-Clover
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140653pMK322 (TOP2A-mAC (Hygro))TOP2A tagging with mAID-Clover
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140654pMK312 (TOP2A CRISPR)TOP2A tagging CRISPR
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140655pMK198 (GAL-OsTIR1)GAL-OsTIR1(WT)
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140656pMK419 (GAL-OsTIR1(F74G))GAL-OsTIR1(F74G)
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140657pMK425 (GAL-OsTIR1(F74A))GAL-OsTIR1(F74A)
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140658pMK427 (OsTIR1(WT) mAID-EGFP-Nluc)OsTIR1(WT)-P2A-mAID-EGFP-Nluc
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140659pMK411 (OsTIR1(F74G) mAID-EGFP-Nluc)OsTIR1(F74G)-P2A-mAID-EGFP-Nluc
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140729pAAV-hSyn-OsTIR1(WT)OsTIR1(WT)-P2A-mAID-EGFP-NES
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140730pAAV-hSyn-OsTIR1(F74G)OsTIR1(F74G)-P2A-mAID-EGFP-NES
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140731pAAV-hSyn-OsTIR1(F74A)OsTIR1(F74A)-P2A-mAID-EGFP-NES
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158621DHC1-AID-Clover donor (Neo)Donor plasmid for tagging DHC1 with AID-Clover
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158622DHC1-AID-Clover donor (Hygro)Donor plasmid for tagging DHC1 with AID-Clover
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158663AAVS1-Tet-OsTIR1(WT)-V5Tet-OsTIR1(WT)-V5 donor for integration at the AAVS1 locus
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159992pMK395 (AAVS1 CMV-OsTIR1(F74A))Expression of OsTIR1(F74A) in human cells
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