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The N-Terminal Domain of cGAS Determines Preferential Association with Centromeric DNA and Innate Immune Activation in the Nucleus.
Gentili M, Lahaye X, Nadalin F, Nader GPF, Puig Lombardi E, Herve S, De Silva NS, Rookhuizen DC, Zueva E, Goudot C, Maurin M, Bochnakian A, Amigorena S, Piel M, Fachinetti D, Londono-Vallejo A, Manel N
Cell Rep. 2019 Feb 26;26(9):2377-2393.e13. doi: 10.1016/j.celrep.2019.01.105.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
127656pTRIP-CMV-Puro-2A-NLS-FLAG-cGASOver-express NLS-FLAG-cGAS
127657pTRIP-CMV-mCherry-FLAG-cGAS E225A/D227AOver-express catalytically inactive mCherry-FLAG-cGAS E225A/D227A
127658pTRIP-CMV-mCherry-53BP1Over-express mCherry-53BP1 fragment to monitor 53BP1 foci.
127660pTRIP-SFFV-EGFP-NLS-FLAG- cGAS E225A/D227AOver-express catalytically inactive EGFP-NLS-FLAG-cGAS E225A/D227A
127662pTRIP-SFFV-EGFP-FLAG-cGAS E225A/D227AOver-express catalytically inactive EGFP-FLAG-cGAS E225A/D227A
127663pTRIP-GFP-IRF3Over-express EGFP-IRF3