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The N-Terminal Domain of cGAS Determines Preferential Association with Centromeric DNA and Innate Immune Activation in the Nucleus.
Gentili M, Lahaye X, Nadalin F, Nader GPF, Puig Lombardi E, Herve S, De Silva NS, Rookhuizen DC, Zueva E, Goudot C, Maurin M, Bochnakian A, Amigorena S, Piel M, Fachinetti D, Londono-Vallejo A, Manel N
Cell Rep. 2019 Feb 26;26(9):2377-2393.e13. doi: 10.1016/j.celrep.2019.01.105.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
127656pTRIP-CMV-Puro-2A-NLS-FLAG-cGASOver-express NLS-FLAG-cGAS
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127657pTRIP-CMV-mCherry-FLAG-cGAS E225A/D227AOver-express catalytically inactive mCherry-FLAG-cGAS E225A/D227A
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127658pTRIP-CMV-mCherry-53BP1Over-express mCherry-53BP1 fragment to monitor 53BP1 foci.
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127659pTRIP-SFFV-EGFP-NLS-FLAG-cGASOver-express EGFP-NLS-FLAG-cGAS
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127660pTRIP-SFFV-EGFP-NLS-FLAG- cGAS E225A/D227AOver-express catalytically inactive EGFP-NLS-FLAG-cGAS E225A/D227A
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127661pTRIP-SFFV-EGFP-FLAG-cGASOver-express EGFP-FLAG-cGAS
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127662pTRIP-SFFV-EGFP-FLAG-cGAS E225A/D227AOver-express catalytically inactive EGFP-FLAG-cGAS E225A/D227A
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127663pTRIP-GFP-IRF3Over-express EGFP-IRF3
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