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pTRIP-SFFV-EGFP-NLS-FLAG- cGAS E225A/D227A
(Plasmid #127660)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 127660 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cGAS
  • Species
    H. sapiens (human)
  • Mutation
    E225A/D227A
  • Entrez Gene
    CGAS (a.k.a. C6orf150, MB21D1, h-cGAS)

Cloning Information

  • Cloning method Unknown

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRIP-SFFV-EGFP-NLS-FLAG- cGAS E225A/D227A was a gift from Nicolas Manel (Addgene plasmid # 127660 ; http://n2t.net/addgene:127660 ; RRID:Addgene_127660)
  • For your References section:

    The N-Terminal Domain of cGAS Determines Preferential Association with Centromeric DNA and Innate Immune Activation in the Nucleus. Gentili M, Lahaye X, Nadalin F, Nader GPF, Puig Lombardi E, Herve S, De Silva NS, Rookhuizen DC, Zueva E, Goudot C, Maurin M, Bochnakian A, Amigorena S, Piel M, Fachinetti D, Londono-Vallejo A, Manel N. Cell Rep. 2019 Feb 26;26(9):2377-2393.e13. doi: 10.1016/j.celrep.2019.01.105. 10.1016/j.celrep.2019.01.105 PubMed 30811988