Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #165419)


Item Catalog # Description Quantity Price (USD)
Plasmid 165419 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pSOUP (helper plasmid for all pGreen based vectors)
  • Backbone manufacturer
    Hellens et al,. 2000
  • Backbone size w/o insert (bp) 9274
  • Total vector size (bp) 9274
  • Vector type
    Helper plasmid required for replication for all pGreen based vectors

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 5 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Although it is a high copy plasmid, it grows slightly slower that one expects. Use tetracycline at 5ug/ml.
  • Copy number
    High Copy


  • Gene/Insert name
    Rep A

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Prof. Roger Hellens when he was at the John Innes Centre
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Hellens, R.P., Edwards, E.A., Leyland, N.R. et al. pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation. Plant Mol Biol 42, 819–832 (2000).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSOUP was a gift from John Innes Centre - Crop Transformation and Genome Editing Group & Wendy Harwood (Addgene plasmid # 165419 ; ; RRID:Addgene_165419)
  • For your References section:

    An Efficient Agrobacterium-Mediated Transformation Protocol for Hexaploid and Tetraploid Wheat. Hayta S, Smedley MA, Clarke M, Forner M, Harwood WA. Curr Protoc. 2021 Mar;1(3):e58. doi: 10.1002/cpz1.58. 10.1002/cpz1.58 PubMed 33656289