pSOUP
(Plasmid #165419)

• Purpose: The pSOUP helper plasmid needed for replication of all pGreen (Hellens et al., 2000) based vectors in Agrobacterium
• Depositing Labs: John Innes Centre - Crop Transformation and Genome Editing Group, Wendy Harwood
• Publication: Hayta et al Curr Protoc. 2021 Mar;1(3):e58. doi: 10.1002/cpz1.58.

Backbone

• Vector backbone: pSOUP (helper plasmid for all pGreen based vectors)
• Backbone manufacturer: Hellens et al,. 2000
• Backbone size w/o insert (bp): 9274
• Total vector size (bp): 9274
• Vector type: Helper plasmid required for replication for all pGreen based vectors

Growth in Bacteria

• Bacterial Resistance(s): Tetracycline, 5 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Although it is a high copy plasmid, it grows slightly slower that one expects. Use tetracycline at 5ug/ml.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rep A

Resource Information

• Supplemental Documents:
  • pSoup.gb
• A portion of this plasmid was derived from a plasmid made by: Prof. Roger Hellens when he was at the John Innes Centre
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Hellens, R.P., Edwards, E.A., Leyland, N.R. et al. pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation. Plant Mol Biol 42, 819–832 (2000). https://doi.org/10.1023/A:1006496308160