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AAV.CBA.eGFP.2A.P301L-Tau
(Plasmid #140425)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 140425 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    AAV.CBA.WPRE
  • Backbone size w/o insert (bp) 6468
  • Total vector size (bp) 8570
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    eGFP-2A-Tau(P301L)
  • Species
    H. sapiens (human), Synthetic
  • Mutation
    changed Proline 301 to Leucine
  • GenBank ID
    NM_005910.5
  • Entrez Gene
    MAPT (a.k.a. DDPAC, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, tau-40)
  • Promoter CBA
  • Tag / Fusion Protein
    • eGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (unknown if destroyed)
  • 3′ cloning site EcoRV (unknown if destroyed)
  • 5′ sequencing primer do not have
  • 3′ sequencing primer do not have
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV.CBA.eGFP.2A.P301L-Tau was a gift from Bradley Hyman (Addgene plasmid # 140425 ; http://n2t.net/addgene:140425 ; RRID:Addgene_140425)
  • For your References section:

    Experimental evidence for the age dependence of tau protein spread in the brain. Wegmann S, Bennett RE, Delorme L, Robbins AB, Hu M, McKenzie D, Kirk MJ, Schiantarelli J, Tunio N, Amaral AC, Fan Z, Nicholls S, Hudry E, Hyman BT. Sci Adv. 2019 Jun 26;5(6):eaaw6404. doi: 10.1126/sciadv.aaw6404. eCollection 2019 Jun. 10.1126/sciadv.aaw6404 PubMed 31249873