AAV.CBA.eGFP.2A.P301L-Tau
(Plasmid #140425)

• Purpose: AAV.CBA.eGFP.2A.P301L-Tau propagation reporter P301L-2N4R-hTau
• Depositing Lab: Bradley Hyman
• Publication: Wegmann et al Sci Adv. 2019 Jun 26;5(6):eaaw6404. doi: 10.1126/sciadv.aaw6404. eCollection 2019 Jun.

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Backbone

• Vector backbone: AAV.CBA.WPRE
• Backbone size w/o insert (bp): 6468
• Total vector size (bp): 8570
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: eGFP-2A-Tau(P301L)
• Species: H. sapiens (human), Synthetic
• Mutation: changed Proline 301 to Leucine
• GenBank ID: NM_005910.5
• Entrez Gene: MAPT (a.k.a. DDPAC, FTD1, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40)
• Promoter: CBA
• Tag / Fusion Protein:
  • eGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xho1 (unknown if destroyed)
• 3′ cloning site: EcoRV (unknown if destroyed)
• 5′ sequencing primer: do not have
• 3′ sequencing primer: do not have

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry