pAAV-FLEX-P301L Tau
(Plasmid
#176267)
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PurposeThis AAV plasmid vector expresses human 2N4R microtubule-associated protein tau with P301L mutation.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 176267 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAAV-EF1a-FLEX-mCherry
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Backbone manufactureraddgene
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFlex-P301L tau
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Entrez GeneMAPT (a.k.a. DDPAC, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Mlu1 (unknown if destroyed)
- 3′ cloning site Nhel (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-FLEX-P301L Tau was a gift from Tsuneya Ikezu (Addgene plasmid # 176267 ; http://n2t.net/addgene:176267 ; RRID:Addgene_176267) -
For your References section:
Cre-inducible Adeno Associated Virus-mediated Expression of P301L Mutant Tau Causes Motor Deficits and Neuronal Degeneration in the Substantia Nigra. You Y, Botros MB, Enoo AAV, Bockmiller A, Herron S, Delpech JC, Ikezu T. Neuroscience. 2019 Dec 1;422:65-74. doi: 10.1016/j.neuroscience.2019.10.001. Epub 2019 Nov 2. 10.1016/j.neuroscience.2019.10.001 PubMed 31689387
