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Live-cell measurements of kinase activity in single cells using translocation reporters.
Kudo T, Jeknic S, Macklin DN, Akhter S, Hughey JJ, Regot S, Covert MW
Nat Protoc. 2018 Jan;13(1):155-169. doi: 10.1038/nprot.2017.128. Epub 2017 Dec 21.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
90227pLentiPGK Puro DEST ERKKTRCloverLentiviral vector to express ERK KTR mClover under PGK promoter (With Puromycin Resistance)
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90229pLentiPGK Hygro DEST ERKKTRmCerulean3Lentiviral vector to express ERK KTR mCerulean3 under PGK promoter (With Hygromycin Resistance)
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90231pLentiPGK Blast DEST ERKKTRmRuby2Lentiviral vector to express ERK KTR mRuby2 under PGK promoter (With Blasticidin Resistance)
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90232pLentiPGK Puro DEST JNKKTRmCerulean3Lentiviral vector to express JNK KTR mCerulean3 under PGK promoter (With Blasticidin Resistance)
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90234pLentiPGK Hygro DEST H2B-mCerulean3Lentiviral vector to express H2B-mCerulean3 under PGK promoter (With Hygromycin Resistance)
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90236pLentiPGK Hygro DEST H2B-mRuby2Lentiviral vector to express H2B-mRuby2 under PGK promoter (With Hygromycin Resistance)
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90237pLentiPGK DEST H2B-iRFP670Lentiviral vector to express H2B-iRFP670 under PGK promoter
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90238pLentiPGK Puro DEST JNKKTR AA CloverLentiviral vector to express JNK KTR AA (mutant) mClover under PGK promoter (With Puromycin Resistance)
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90239pLentiPGK Puro DEST JNKKTR EE CloverLentiviral vector to express JNK KTR EE (mutant) mClover under PGK promoter (With Puromycin Resistance)
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90240pLentiPGK Puro DEST JNKKTR AE CloverLentiviral vector to express JNK KTR AE (mutant) mClover under PGK promoter (With Puromycin Resistance)
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