pLentiPGK Puro DEST JNKKTR EE Clover
PurposeLentiviral vector to express JNK KTR EE (mutant) mClover under PGK promoter (With Puromycin Resistance)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||90239||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepLENTI PGK Puro DEST (w529-2)
Backbone manufacturerEric Campeau Lab (Addgene Plasmid 19068)
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin (50 ug/mL)
Gene/Insert nameJNK Kinase Translocation Reporter (EE mutant)
SpeciesH. sapiens (human), M. musculus (mouse)
- Promoter PGK
/ Fusion Protein
- mClover (C terminal on insert)
- Cloning method Gateway Cloning
- 5′ sequencing primer ACCGAATCACCGACCTCTCT
- 3′ sequencing primer GCAGCGTATCCACATAGCG (Common Sequencing Primers)
JNKKTR EE has an M11I mutation compared to WT JNKKTR. The mutation does not alter plasmid function as described in the associated publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLentiPGK Puro DEST JNKKTR EE Clover was a gift from Markus Covert (Addgene plasmid # 90239 ; http://n2t.net/addgene:90239 ; RRID:Addgene_90239)
For your References section:Live-cell measurements of kinase activity in single cells using translocation reporters. Kudo T, Jeknic S, Macklin DN, Akhter S, Hughey JJ, Regot S, Covert MW. Nat Protoc. 2018 Jan;13(1):155-169. doi: 10.1038/nprot.2017.128. Epub 2017 Dec 21. 10.1038/nprot.2017.128 PubMed 29266096