168,234 results

1. pSAM_AraC

   Plasmid

   #91569

   Purpose

   Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.

   Depositor

   Harry Mobley

   Article

   Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.

   Insert

   AraC/PBAD
   

   Expression

   Bacterial

   Available Since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL3-ELAM-luc

   Plasmid

   #13029

   Depositor

   Doug Golenbock

   Insert

   ELAM (SELE Human)
   

   Use

   Luciferase

   Tags

   Luc

   Expression

   Mammalian

   Available Since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pILGFPI7

   Plasmid

   #83555

   Purpose

   Used to evaluate the expression output of CUP1 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   CUP1 promoter-yEGFP
   

   Expression

   Yeast

   Promoter

   CUP1

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221-SLC32A1_STOP

   Plasmid

   #161278

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC32A1 (SLC32A1 Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 15, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pTRE-tdTomato

   Plasmid

   #50798

   Purpose

   Expression of tdTomato under the control of the TRE-promoter

   Depositor

   Connie Cepko

   Article

   Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

   Insert

   TREtight driven tdTomato fluorescent protein
   

   Use

   Mutli-species

   Promoter

   TREtight

   Available Since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-ePhi29 (LM2993)

   Plasmid

   #208959

   Purpose

   A variant CE1 construct with ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-ePhi29-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); ePhi29(-exo)(D169A;M8R/V51A/M97T/…

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. TOP-H2B-YFP-DD

   Plasmid

   #96891

   Purpose

   Reporter to monitor translation of 5'TOP mRNA

   Depositor

   Tobias Meyer

   Article

   Han et al Nat Methods. 2014 Jan;11(1):86-93. doi:

   Insert

   TOP-H2B-YFP-DD
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF2399

   Plasmid

   #144334

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZFPM1 (ZFPM1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-NLS-Cas9-6xHis

   Plasmid

   #62934

   Purpose

   Expression of NLS-Cas9-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   NLS-Cas9-6xHis
   

   Tags

   6xHis tag and NLS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmCherry-EXO1a

    Plasmid

    #111629

    Purpose

    Expresses human WT Exonuclease 1a (EXO1a) tagged with mCherry in mammalian cells

    Depositor

    Marco Muzi-Falconi

    Article

    Sertic et al Proc Natl Acad Sci U S A. 2011 Aug 16;10

    Insert

    Exonuclease 1a (EXO1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-Best1-sCD200

    Plasmid

    #164424

    Purpose

    AAV plasmid expressing soluble CD200 in retinal pigment epithelium

    Depositor

    Connie Cepko

    Article

    Wang et al Proc Natl Acad Sci U S A. 2019 May 14;11

    Insert

    CD200 (Cd200 Mouse)
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    deleted terminal 40 amino acids

    Promoter

    Best1

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV CAGG eGFP

    Plasmid

    #107707

    Purpose

    express eGFP in variety of cell types

    Depositor

    Troy Margrie

    Article

    Velez-Fort et al Neuron. 2018 Apr 4;98(1):179-191.e6. doi

    Insert

    eGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAGG

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. PMP34-mApple

    Plasmid

    #214417

    Purpose

    Peroxisome localization of PMP34 fused to mApple

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    PMP34 (SLC25A17 Human)
    

    Tags

    mApple

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. L13-Arl13bGFP

    Plasmid

    #40879

    Purpose

    3rd generation transfer vector

    Depositor

    Tamara Caspary

    Article

    Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

    Insert

    Arl13b (Arl13b Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBIG2abc

    Plasmid

    #80617

    Purpose

    pBIG2abc - Step2 vector abc (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. MAC_C_PTH2

    Plasmid

    #111674

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    PTH2 (PTRH2 Human)
    

    Expression

    Mammalian

    Available Since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Flag-mFoxJ1

    Plasmid

    #122965

    Purpose

    Expresses Flag-tagged mouse FoxJ1 in mammalian cells

    Depositor

    FENG-QIAN Li, Ken-Ichi Takemaru

    Insert

    mouse FoxJ1 (Foxj1 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCIneoMyc UNC119B

    Plasmid

    #128333

    Purpose

    Mammalian expression vector of Myc-tagged human UNC119B

    Depositor

    Yutaka Hata

    Article

    Iwasa et al Cancer Sci. 2018 Sep;109(9):2767-2780. d

    Insert

    UNC119B (UNC119B Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBIG2abcde

    Plasmid

    #80619

    Purpose

    pBIG2abcde - Step2 vector abcde (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBIG2abcd

    Plasmid

    #80618

    Purpose

    pBIG2abcd - Step2 vector abcd (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only