Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

163,977 results

Showing: 3481 - 3500 of 163977 results

  2. pET21b(+)-Is-PETase-W159H-S238F

    Plasmid
    #112203
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12
    Depositor
    Gregg Beckham, Christopher Johnson
    Article
    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115
    Insert
    PETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12
    Use
    Tags
    6XHIS
    Expression
    Bacterial
    Mutation
    W159H, S238F
    Promoter
    N/A
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    112203-plasmid-map-sequence-id-473446

    Enlarge

  3. pEGFP GR

    Plasmid
    #47504
    Purpose
    Expresses glucocorticoid receptor in mammalian cells
    Depositor
    Alice Wong
    Insert
    Glucocorticoid receptor (NR3C1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only
    47504-plasmid-map-sequence-id-223484

    Enlarge

  8. pcDNA3.1_FASN

    Plasmid
    #107138
    Purpose
    His tagged human FASN cloned in pcDNA3.1 vector for expression in mammalian cells
    Depositor
    Brijesh Kumar Singh
    Insert
    Fatty acid synthase (FASN Human)
    Use
    Tags
    His
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107138-plasmid-map-sequence-id-423159

    Enlarge

  11. p_m16S

    Plasmid
    #160549
    Purpose
    Used for mycoplasma detection by qPCR. 16S insert truncated internally from the strain Mycoplasma capricolum subsp. capricolum strain California Kid. ATB: Carbenicilline
    Depositor
    Denis Gerlier
    Article
    Jean et al PLoS One. 2017 Feb 22;12(2):e0172358. do
    Insert
    16S rDNA fragment from M. capricolum subsp. capricolum strain California Kid (gi_83283139)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    160549-plasmid-map-sequence-id-319414

    Enlarge

  13. pCJ136

    Plasmid
    #162665
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162665-plasmid-map-sequence-id-321451

    Enlarge

  15. pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (AAV Retrograde)

    Viral Prep
    #55645-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (#55645). In addition to the viral particles, you will also receive purified pAAV-hSyn Con/Fon hChR2(H134R)-EYFP plasmid DNA. Synapsin-driven, Cre-dependent and Flp-dependent Channelrhodopsin-EYFP expression for optogenetic activation. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:
    Promoter
    Syn
    Tags
    EYFP (Cre- and Flp-dependent)
    Available since
    Dec. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 3481 - 3500 of 163977 results