AMPK governs lineage specification through Tfeb-dependent regulation of lysosomes.
Young NP, Kamireddy A, Van Nostrand JL, Eichner LJ, Shokhirev MN, Dayn Y, Shaw RJ
Genes Dev. 2016 Mar 1;30(5):535-52. doi: 10.1101/gad.274142.115.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 79004 | Cas9-GFP_sg_mAMPKa1 | Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 1. |
| 79005 | Cas9-GFP_sg_mAMPKa2 | Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 2. |
| 79006 | Cas9-GFP_sg_mTfeb | Expresses WT Cas9 and GFP, along with a sgRNA to mouse Tfeb. |
| 79009 | pCIP | For expression in mouse ESCs or other eukaryotic cells. Contains pCAGGS-IRES-Puro-bpA, with a NheI-MfeI cloning site in between pCAGGS and IRES. |
| 79010 | pCIP-AMPKa1_WT | For mammalian expression of WT AMPK alpha1. |
| 79011 | pCIP-AMPKa1_KD | For mammalian expression of a kinase dead (K47R) version of AMPK alpha1. |
| 79012 | pCIP-GFP | For mammalian expression of GFP. |
| 79013 | pCIP-caTfeb | For mammalian expression of a shortened, constitutively active version of Tfeb. |
| 79014 | pCIP-Flag-TfebAA | For mammalian expression of a Flag-tagged, constitutively active version (S142A, S211A) of Tfeb. |