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AMPK governs lineage specification through Tfeb-dependent regulation of lysosomes.
Young NP, Kamireddy A, Van Nostrand JL, Eichner LJ, Shokhirev MN, Dayn Y, Shaw RJ
Genes Dev. 2016 Mar 1;30(5):535-52. doi: 10.1101/gad.274142.115.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
79004Cas9-GFP_sg_mAMPKa1Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 1.
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79005Cas9-GFP_sg_mAMPKa2Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 2.
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79006Cas9-GFP_sg_mTfebExpresses WT Cas9 and GFP, along with a sgRNA to mouse Tfeb.
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79009pCIPFor expression in mouse ESCs or other eukaryotic cells. Contains pCAGGS-IRES-Puro-bpA, with a NheI-MfeI cloning site in between pCAGGS and IRES.
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79010pCIP-AMPKa1_WTFor mammalian expression of WT AMPK alpha1.
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79011pCIP-AMPKa1_KDFor mammalian expression of a kinase dead (K47R) version of AMPK alpha1.
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79012pCIP-GFPFor mammalian expression of GFP.
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79013pCIP-caTfebFor mammalian expression of a shortened, constitutively active version of Tfeb.
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79014pCIP-Flag-TfebAAFor mammalian expression of a Flag-tagged, constitutively active version (S142A, S211A) of Tfeb.
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