PurposeAdeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct. Note that an improved version is available: Addgene plasmid# 92307. See comments for details
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||68837||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5238
- Total vector size (bp) 7446
Vector typeMammalian Expression, AAV, Synthetic Biology
Growth in Bacteria
Growth instructionsStbl4 is also appropriate for growth.
Copy numberHigh Copy
Gene/Insert nameAncestral AAV Capsid Anc80L65
Insert Size (bp)2208
- Promoter Rep2
- Cloning method Unknown
- 5′ sequencing primer M13rev (Common Sequencing Primers)
An improved version of this plasmid from the Vandenberghe Lab is available. Please see pAnc80L65AAP (Addgene plasmid# 92307).
This plasmid, pAnc80L65 (#68837), requires cotransfection with a plasmid expressing AAP. For efficient viral vector production without cotransfecting AAP, use pAnc80L65AAP (Addgene plasmid# 92307)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAnc80L65 was a gift from Luk Vandenberghe (Addgene plasmid # 68837 ; http://n2t.net/addgene:68837 ; RRID:Addgene_68837)
For your References section:In Silico Reconstruction of the Viral Evolutionary Lineage Yields a Potent Gene Therapy Vector. Zinn E, Pacouret S, Khaychuk V, Turunen HT, Carvalho LS, Andres-Mateos E, Shah S, Shelke R, Maurer AC, Plovie E, Xiao R, Vandenberghe LH. Cell Rep. 2015 Jul 28. pii: S2211-1247(15)00759-7. doi: 10.1016/j.celrep.2015.07.019. 10.1016/j.celrep.2015.07.019 PubMed 26235624