PurposeThis plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the 7M8 capsid protein.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||64839||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerUniversity of North Carolina Dr. Samulski
- Backbone size w/o insert (bp) 5648
- Total vector size (bp) 8209
Modifications to backbonereplaced AAV2 cap with 7M8 cap
Growth in Bacteria
Growth Strain(s)NEB Stable
Gene/Insert name7M8 cap
SpeciesAdeno-associated virus - 2
Insert Size (bp)2238
Mutation7mer insertion in the AAV2 cap
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer GCGGAAGCTTCGATCAACTACGC
- 3′ sequencing primer gtgcggcaaagtttgcttcc (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Discrepancies found during QC have no functional consequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:7M8 was a gift from John Flannery & David Schaffer (Addgene plasmid # 64839 ; http://n2t.net/addgene:64839 ; RRID:Addgene_64839)
For your References section:In vivo-directed evolution of a new adeno-associated virus for therapeutic outer retinal gene delivery from the vitreous. Dalkara D, Byrne LC, Klimczak RR, Visel M, Yin L, Merigan WH, Flannery JG, Schaffer DV. Sci Transl Med. 2013 Jun 12;5(189):189ra76. doi: 10.1126/scitranslmed.3005708. 10.1126/scitranslmed.3005708 PubMed 23761039