PurposeMammalian expression plasmid of OptoSTIM1 (optogenetically-activated STIM1 protein)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||70159||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 7657
Modifications to backboneEGFP gene was removed and re-inserted at restriction enzyme sites NheI and AgeI.
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human), A. thaliana (mustard weed)
Insert Size (bp)3663
Entrez GeneSTIM1 (a.k.a. D11S4896E, GOK, IMD10, STRMK, TAM, TAM1)
- Promoter CMV
/ Fusion Protein
- EGFP (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40-pAR (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV-OptoSTIM1 was a gift from Won Do Heo (Addgene plasmid # 70159 ; http://n2t.net/addgene:70159 ; RRID:Addgene_70159)
For your References section:Optogenetic control of endogenous Ca channels in vivo. Kyung T, Lee S, Kim JE, Cho T, Park H, Jeong YM, Kim D, Shin A, Kim S, Baek J, Kim J, Kim NY, Woo D, Chae S, Kim CH, Shin HS, Han YM, Kim D, Heo WD. Nat Biotechnol. 2015 Sep 14. doi: 10.1038/nbt.3350. 10.1038/nbt.3350 PubMed 26368050