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pRK5 TGF beta type I receptor (T202D) Flag
(Plasmid #14833)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14833 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRK5 modified with flag
  • Backbone manufacturer
    Genentech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TGF beta type I receptor
  • Alt name
    TGFb type I receptor
  • Alt name
    Tgfbr1
  • Alt name
    ALK5
  • Species
    R. norvegicus (rat)
  • Mutation
    Threonine 202 mutated to aspartate. Constitutively active mutant.
  • Entrez Gene
    Tgfbr1 (a.k.a. Alk5, Skr4, Tgfr-1, tbetaR-I)
  • Tag / Fusion Protein
    • flag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

See Author's map for more information.

A 9bp linker was added directly upstream of the GS domain. The linker encodes the amino acids GSM. The additional sequence does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5 TGF beta type I receptor (T202D) Flag was a gift from Rik Derynck (Addgene plasmid # 14833 ; http://n2t.net/addgene:14833 ; RRID:Addgene_14833)
  • For your References section:

    Ligand-independent activation of transforming growth factor (TGF) beta signaling pathways by heteromeric cytoplasmic domains of TGF-beta receptors. Feng XH, Derynck R. J Biol Chem. 1996 May 31. 271(22):13123-9. 10.1074/jbc.271.22.13123 PubMed 8662796