pRK5 TGF beta type I receptor Flag
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14831||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepRK5 modified with flag
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameTGF beta type I receptor
Alt nameTGFb type I receptor
SpeciesR. norvegicus (rat)
Entrez GeneTgfbr1 (a.k.a. Alk5, Skr4, Tgfr-1, tbetaR-I)
/ Fusion Protein
- flag (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
See Author's map for more information.
A 9bp linker was added directly upstream of the GS domain. The linker encodes the amino acids GSM. The additional sequence does not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRK5 TGF beta type I receptor Flag was a gift from Rik Derynck (Addgene plasmid # 14831 ; http://n2t.net/addgene:14831 ; RRID:Addgene_14831)
For your References section:Ligand-independent activation of transforming growth factor (TGF) beta signaling pathways by heteromeric cytoplasmic domains of TGF-beta receptors. Feng XH, Derynck R. J Biol Chem. 1996 May 31. 271(22):13123-9. 10.1074/jbc.271.22.13123 PubMed 8662796