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Rick Tarleton Lab Plasmids


The Rick Tarleton Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Evaluation of high efficiency gene knockout strategies for Trypanosoma cruzi. Xu D, Brandan CP, Basombrio MA, Tarleton RL BMC Microbiol. 2009 May 11;9:90. doi: 10.1186/1471-2180-9-90.
In vitro and in vivo high-throughput assays for the testing of anti-Trypanosoma cruzi compounds. Canavaci AM, Bustamante JM, Padilla AM, Perez Brandan CM, Simpson LJ, Xu D, Boehlke CL, Tarleton RL PLoS Negl Trop Dis. 2010 Jul 13;4(7):e740. doi: 10.1371/journal.pntd.0000740.
Trasgenically expresses firefly luciferase in T. cruzi (unpublished)
CRISPR-Cas9-Mediated Single-Gene and Gene Family Disruption in Trypanosoma cruzi. Peng D, Kurup SP, Yao PY, Minning TA, Tarleton RL MBio. 2014 Dec 30;6(1). pii: e02097-14. doi: 10.1128/mBio.02097-14.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
47975pTrex-Neo-tdTomatotdTomato (Synthetic)Trypanasoma cruzi expressionIn vitro and in vivo high-throughput assays for the testing of anti-Trypanosoma cruzi compounds. PLoS Negl Trop Dis. 2010 Jul 13;4(7):e740. doi: 10.1371/journal.pntd.0000740. Trasgenically expresses tandem tdtomato fluorescent protein in T. cruzi pTrex-Neo Add to Cart
48157pLew90β-GW/T7/PARP SAS/luciferase/Aldolase 3′UTRluciferase (Other)Luciferase ; T. cruzi expressionIn vitro and in vivo high-throughput assays for the testing of anti-Trypanosoma cruzi compounds. PLoS Negl Trop Dis. 2010 Jul 13;4(7):e740. doi: 10.1371/journal.pntd.0000740. Transgenically expresses firefly luciferase protein in T. cruzi pLew90β-T7-GW-Neo Add to Cart
48336pTrex-Luc-Neofirely luciferase (Other)Trypanasoma cruzi expression Trasgenically expresses firefly luciferase in T. cruzi (unpublished) Trasgenically expresses firefly luciferase in T. cruzi with neomycin selection pTrex Add to Cart
48337pTrex-Luc-Phleofirely luciferase (Other)Trypanasoma cruzi expression Trasgenically expresses firefly luciferase in T. cruzi (unpublished) Trasgenically expresses firefly luciferase in T. cruzi with phleomycin selection pTrex Add to Cart
48993pDONR221-Neoaminoglycoside 3' phosphotransferaseGateway attB1 & attB2Evaluation of high efficiency gene knockout strategies for Trypanosoma cruzi. BMC Microbiol. 2009 May 11;9:90. doi: 10.1186/1471-2180-9-90. Gateway adapted transfer of Neomycin resistance gene pDONR221 Add to Cart
48994pDONR221-Hyghygromycin B phosphotransferaseGateway attB1 & attB2Evaluation of high efficiency gene knockout strategies for Trypanosoma cruzi. BMC Microbiol. 2009 May 11;9:90. doi: 10.1186/1471-2180-9-90. Gateway adapted transfer of Hygromycin resistance gene pDONR221 Add to Cart
48995pDONR221-PheloSh bleGateway attB1 & attB2Evaluation of high efficiency gene knockout strategies for Trypanosoma cruzi. BMC Microbiol. 2009 May 11;9:90. doi: 10.1186/1471-2180-9-90. Gateway adapted transfer of Phleomycin resistance gene pDONR221 Add to Cart
62543pTrex-b-NLS-hSpCas9NLS-hSpCas9CRISPR ; Trypanosoma cruzi expressionCRISPR-Cas9-Mediated Single-Gene and Gene Family Disruption in Trypanosoma cruzi. MBio. 2014 Dec 30;6(1). pii: e02097-14. doi: 10.1128/mBio.02097-14. Expression of Cas9 in T. cruzi pTrex-b Add to Cart
62544pTrex-n-eGFPeGFP(1)Trypanosoma cruzi expression of eGFP (2) genome integration of eGFPCRISPR-Cas9-Mediated Single-Gene and Gene Family Disruption in Trypanosoma cruzi. MBio. 2014 Dec 30;6(1). pii: e02097-14. doi: 10.1128/mBio.02097-14. Expression of eGFP in T. cruzi. The NheI-linearized form of this plasmid could be efficiently integrated in to T. cruzi genome pTrex-n Add to Cart