pDONR207 SARS-CoV-2 M
PurposeGateway-compatible Entry vector, with insert of the membrane (M) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||141274||Standard format: Plasmid sent in bacteria as agar stab||1||$85 *|
|Cloning Grade DNA||141274-DNA.cg||2 µg of cloning grade DNA in Tris buffer||1||$105 *|
* Login to view industry pricing.
- Backbone size w/o insert (bp) 5585
Vector typeGateway-compatible Entry vector
Growth in Bacteria
Bacterial Resistance(s)Gentamicin, 10 μg/mL
Alt nameGene ID 43740571
SpeciesSARS-CoV-2 isolate Wuhan-Hu-1
Insert Size (bp)669
MutationMany synonymous changes due to codon optimization
Entrez GeneM (a.k.a. GU280_gp05)
- Cloning method Gateway Cloning
- 5′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
- Zeocin® is an InvivoGen trademark.
Information for Cloning Grade DNA (Catalog # 141274-DNA.cg) ( Back to top )
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $105 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDONR207 SARS-CoV-2 M was a gift from Fritz Roth (Addgene plasmid # 141274 ; http://n2t.net/addgene:141274 ; RRID:Addgene_141274)
For your References section:A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions. Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale M, Raught B, Jacob Y, Roth FP. G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 10.1534/g3.120.401554. 10.1534/g3.120.401554 PubMed 32763951