A versatile one-step CRISPR-Cas9 based approach to plasmid-curing.
Lauritsen I, Porse A, Sommer MOA, Norholm MHH
Microb Cell Fact. 2017 Aug 2;16(1):135. doi: 10.1186/s12934-017-0748-z. PubMed
Lauritsen I, Porse A, Sommer MOA, Norholm MHH
Microb Cell Fact. 2017 Aug 2;16(1):135. doi: 10.1186/s12934-017-0748-z. PubMed
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
92050 | pFREE | Allows inducible expression of gRNAs and Cas9 for plasmid curing. |
92051 | pFREE_Amp | Allows inducible expression of gRNAs and Cas9 for plasmid curing. |
92052 | pFREE_Cm | Allows inducible expression of gRNAs and Cas9 for plasmid curing. |
92053 | pFREE_Zeo | Allows inducible expression of gRNAs and Cas9 for plasmid curing. |
92054 | pFREE-RK2 | Allows inducible expression of gRNAs and Cas9 for plasmid curing as well as self-curing via a temperature sensitive RK2 replicon. |