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A versatile one-step CRISPR-Cas9 based approach to plasmid-curing.
Lauritsen I, Porse A, Sommer MOA, Norholm MHH
Microb Cell Fact. 2017 Aug 2;16(1):135. doi: 10.1186/s12934-017-0748-z.
PubMed

Plasmids from Article

ID Plasmid Purpose  
92050pFREEAllows inducible expression of gRNAs and Cas9 for plasmid curing.
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92051pFREE_AmpAllows inducible expression of gRNAs and Cas9 for plasmid curing.
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92052pFREE_CmAllows inducible expression of gRNAs and Cas9 for plasmid curing.
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92053pFREE_ZeoAllows inducible expression of gRNAs and Cas9 for plasmid curing.
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92054pFREE-RK2Allows inducible expression of gRNAs and Cas9 for plasmid curing as well as self-curing via a temperature sensitive RK2 replicon.
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