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(Plasmid #92050)


Item Catalog # Description Quantity Price (USD)
Plasmid 92050 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
  • Promoter ptet

Cloning Information for Gene/Insert 1

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer AAAGTCTGGTTATAACC
  • 3′ sequencing primer TACCGGTTTATTGACTACC
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    gRNA array
  • Species
  • Promoter pRhamBAD

Cloning Information for Gene/Insert 2

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer gccacaattcagcaaattgtgaac
  • 3′ sequencing primer GTGCCGATATCTAAGCCTATTGAG
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFREE was a gift from Morten Norholm (Addgene plasmid # 92050 ; ; RRID:Addgene_92050)
  • For your References section:

    A versatile one-step CRISPR-Cas9 based approach to plasmid-curing. Lauritsen I, Porse A, Sommer MOA, Norholm MHH. Microb Cell Fact. 2017 Aug 2;16(1):135. doi: 10.1186/s12934-017-0748-z. 10.1186/s12934-017-0748-z [pii] PubMed 28764701