169,450 results
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Plasmid#208717PurposeExpresses the green 5-HT sensor GRAB_g5-HT3.0 in neuronsDepositorHas ServiceAAV1, AAV2, and AAV8InsertGreen fluorescent 5-HT sensor GRAB_g5-HT3.0
UseAAVPromoterhSynAvailable SinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET30a-AlkB-D135S
Plasmid#79051PurposeExpress mutant AlkB protein (D135S) in E. coli BL21(DE3)DepositorInsertAlkB (alkB E.coli)
TagsHis tagExpressionBacterialMutationA truncated AlkB with deletion of the amino (N)-t…PromoterT7Available SinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lamp1-mTurquoise2
Plasmid#98828PurposeIn vivo visualization of lysosomes (can be used for colocalization studies)DepositorInsertLamp1
TagsmTurquoise2ExpressionMammalianPromoterCMVAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV IRES Luciferase
Plasmid#18760DepositorTypeEmpty backboneUseLuciferase and RetroviralExpressionMammalianAvailable SinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Actin-7
Plasmid#56421PurposeLocalization: Actin, Excitation: 488, Emission: 507DepositorAvailable SinceDec. 23, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSB90 - pAGM4723_VirGN54D
Plasmid#123187PurposeGolden Gate (MoClo; Weber et al., 2011) Level 2 acceptor vector with increased virulence in AgrobacteriaDepositorTypeEmpty backboneUseLevel 2 acceptor vector for expression in plantsExpressionPlantMutationVirGN54D for increase virulenceAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-smURFP
Plasmid#80349PurposeLentiviral transfer vector under the CMV promoter that expresses smURFP.DepositorInsertsmURFP
UseLentiviralPromoterCMVAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-BH
Plasmid#60499PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of BFP, rtTA and hygromycin resistance geneDepositorTypeEmpty backboneUseTransposonExpressionMammalianPromoterTCE & RPBSAAvailable SinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUCC001
Plasmid#124451PurposeMulti-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targetsDepositorInsertHH-BsaI
ExpressionYeastMutationgRNA expression cassette from pUDP002 modified to…PromoterTDH3 promoter from S.cerevisiae (already present …Available SinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Puro-BRAF-V600E
Plasmid#15269PurposeRetroviral expression of BRAF V600E (the V600E mutation has been found in cancer patients, confers increased kinase activity, and is transforming)DepositorInsertBRAF (BRAF Human)
UseRetroviralTagsHis and MycExpressionMammalianMutationValine 600 to Glutamate (mutation has been found …Available SinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
TRE-mClover3
Plasmid#214922Purposeexpression vector control - contitutive expression of mClover3 aloneDepositorInsertmClover3
UseLentiviralAvailable SinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF142_U6-sgRNA
Plasmid#225960PurposeU6-sgRNA (recipient). Expresses U6-sgRNA (recipient) for VLP production. This is a recipient vector for cloning of specific SpyCas9 sgRNAs.DepositorInsertU6-sgRNA (recipient)
ExpressionMammalianAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMG36E
Plasmid#133853PurposeExpression vectorDepositorTypeEmpty backboneExpressionBacterialPromoterP32Available SinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF1479
Plasmid#143504PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGD-P1/HC-Pro
Plasmid#196328Purpose35S promoter-driven expression of the tobacco etch virus P1/HC-Pro RNA silencing suppressorDepositorInserttobacco etch virus P1/HC-Pro
ExpressionPlantPromoterduplicated cauliflower mosaic virus 35S promoterAvailable SinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-FKBP:mCherry-KRas4Bc30
Plasmid#72900PurposeMembrane-targeted mCherry-tagged recruiter constructDepositorInsertGTPase K-Ras (KRAS Human)
UseLentiviralTagsFKBP and mCherryExpressionMammalianMutationamino acids 158..188 (fragment 1..157 deleted)Available SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-hChR2-H134R-tdTomato (AAV Retrograde)
Viral Prep#28017-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV-CAG-hChR2-H134R-tdTomato (#28017). In addition to the viral particles, you will also receive purified AAV-CAG-hChR2-H134R-tdTomato plasmid DNA. Humanized channelrhodopsin H134R mutant fused to tdTomato, under the control of the CAG promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagstdTomatoAvailable SinceJune 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.S
Plasmid#103006Purposenon-standard AAV2 rep-AAV-PHP.S cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.S VP1 gene
UseAAVExpressionMammalianPromoterp41Available SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
Nb-gp41-GFP (MoonTag-Nb-GFP)
Plasmid#128602PurposeExpresses nanobody-2H10 fused to super folded GFP (sfGFP) in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).DepositorInsertNanobody_gp41
UseLentiviralTagsGB1 and sfGFPPromoterSFFVAvailable SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
mNeongreen-2xrGBD
Plasmid#129624PurposeVisualization of active RhoADepositorInsertmNeongreen-2xrGBD
ExpressionMammalianPromoterCMVdelAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only