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An optimized fluorescent probe for visualizing glutamate neurotransmission.
Marvin JS, Borghuis BG, Tian L, Cichon J, Harnett MT, Akerboom J, Gordus A, Renninger SL, Chen TW, Bargmann CI, Orger MB, Schreiter ER, Demb JB, Gan WB, Hires SA, Looger LL
Nat Methods. 2013 Feb;10(2):162-70. doi: 10.1038/nmeth.2333. Epub 2013 Jan 13.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
41732pCMV(MinDis).iGluSnFRa single-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed.
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41733pRSET.GltI253-cpGFP.L1LV/L2NPa single-wavelength intracellular glutamate sensor constructed from E. coli Gltl and cpGFP. For bacterial cytoplasmic expression
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98929pAAV.hSyn.iGluSnFr.WPRE.SV40 AAV expression of glutamate sensor from human synapsin promoter
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98930pENN.AAV.GFAP.iGluSnFr.WPRE.SV40 AAV expression of glutamate sensor from GFAP promoter
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98931pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40 Cre dependent AAV expression of glutamate sensor from human synapsin promoter
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98932pAAV.CAG.Flex.iGluSnFr.WPRE.SV40Cre dependent AAV expression of glutamate sensor from GFAP promoter
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