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A light-inducible CRISPR-Cas9 system for control of endogenous gene activation.
Polstein LR, Gersbach CA
Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
60551pcDNA3.1-CibN-dCas9Expresses CibN-dCas9 in mammalian cells
60552pcDNA3.1-dCas9-CibNExpresses dCas9-CibN in mammalian cells
60553pcDNA3.1-CibN-dCas9-CibNExpresses CibN-dCas9-CibN in mammalian cells
60554pcDNA3.1-Cry2FL-VP64Expresses Cry2FL-VP64 in mammalian cells
60718pGL3-Basic-8x-gRNA-eGFPeGFP reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation
60719gRNA-eGFP-ReporterExpresses guide RNA used to activate pGL3-Basic-8x-gRNA-eGFP reporter.

Antibodies from Article