|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16059||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneTCF3 (a.k.a. AGM8, E2A, E47, ITF1, TCF-3, VDIR, bHLHb21, p75)
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3 hE47 was a gift from Robert Benezra (Addgene plasmid # 16059 ; http://n2t.net/addgene:16059 ; RRID:Addgene_16059)
For your References section:Overexpression of Id protein inhibits the muscle differentiation program: in vivo association of Id with E2A proteins. Jen Y, Weintraub H, Benezra R. Genes Dev. 1992 Aug . 6(8):1466-79. 10.1101/gad.6.8.1466 PubMed 1644289