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pcDNA/Myc TCF4
(Plasmid #16512)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16512 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TCF4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2400
  • Entrez Gene
    TCF7L2 (a.k.a. TCF-4, TCF4)
  • Tag / Fusion Protein
    • Myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site See map (not destroyed)
  • 3′ cloning site See map (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

The TCF4 in this construct has a Q27R mutation. It is not known to affect the function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA/Myc TCF4 was a gift from Bert Vogelstein (Addgene plasmid # 16512 ; http://n2t.net/addgene:16512 ; RRID:Addgene_16512)
  • For your References section:

    Constitutive transcriptional activation by a beta-catenin-Tcf complex in APC-/- colon carcinoma. Korinek V, Barker N, Morin PJ, van Wichen D, de Weger R, Kinzler KW, Vogelstein B, Clevers H. Science. 1997 Mar 21. 275(5307):1784-7. 10.1126/science.275.5307.1784 PubMed 9065401