p3xFLAG-mE47
(Plasmid #34585)

• Depositing Lab: Celeste Nelson
• Publication: Lee et al EMBO J. 2011 May 24;30(13):2662-74. doi: 10.1038/emboj.2011.159.

Backbone

• Vector backbone: p3xFlag-CMV-7.1
• Backbone manufacturer: Sigma
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: E47
• Alt name: Tcf3
• Species: M. musculus (mouse)
• Insert Size (bp): 2000
• Entrez Gene: Tcf3 (a.k.a. A1, ALF2, E12, E12/E47, E2A, E47, KA1, ME2, Pan1, Pan2, TCF-3, Tcfe2a, VDIR, bHLHb21)
• Promoter: CMV
• Tag / Fusion Protein:
  • 3xFLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV forward

Resource Information

• Supplemental Documents:
  • p3xFLAG-mmE47_datasheet
• Addgene Notes:
  • Addgene Diagnostic Digest
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry