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Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9.
Li JF, Norville JE, Aach J, McCormack M, Zhang D, Bush J, Church GM, Sheen J
Nat Biotechnol. 2013 Aug;31(8):688-91. doi: 10.1038/nbt.2654.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
52254HBT-pcoCas9transient expression of pcoCas9 gene in plant cells
52255pUC119-gRNACan use a PCR template to assemble new desired guide RNA. Contains an Arabidopsis U6 promoter to drive guide RNA (targeting AtPDS3 gene target site 1) expression with a TTTTTT as terminator.

Antibodies from Article