Skip to main content
Holiday Schedule: Addgene will be closed November 26th & 27th for the Thanksgiving Holiday. Order processing and shipping may be delayed during this week. For questions about your shipment please contact [email protected].
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Genetic screens in human cells using the CRISPR-Cas9 system.
Wang T, Wei JJ, Sabatini DM, Lander ES
Science. 2014 Jan 3;343(6166):80-4. doi: 10.1126/science.1246981. Epub 2013 Dec 12.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
50661pCW-Cas9Doxycycline-inducible lentiviral expression of SpCas9
Loading...
50662pLX-sgRNALentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)
Loading...
51043Human Lentiviral sgRNA Library - Unknown FunctionSub-pool of Sabatini/Lander sgRNA library enriched for gene targets of unknown function.
Loading...
51044Human Lentiviral sgRNA Library - KinasesSub-pool of Sabatini/Lander sgRNA library enriched for kinase targets.
Loading...
51045Human Lentiviral sgRNA Library - Ribosomal ProteinsSub-pool of Sabatini/Lander sgRNA library enriched for ribosomal gene targets.
Loading...
51046Human Lentiviral sgRNA Library - Cell CycleSub-pool of Sabatini/Lander sgRNA library enriched for cell cycle-related targets.
Loading...
51047Human Lentiviral sgRNA Library - NuclearSub-pool of Sabatini/Lander sgRNA library enriched for nuclear targets.
Loading...
51048Human Lentiviral sgRNA Library - ControlSub-pool of Sabatini/Lander sgRNA library enriched for control targets.
Loading...