Rc/CMV cyclin A
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8959||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5542
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namecyclin A
SpeciesH. sapiens (human)
Insert Size (bp)1900
Entrez GeneCCNA2 (a.k.a. CCN1, CCNA)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
See author's map.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Rc/CMV cyclin A was a gift from Bob Weinberg (Addgene plasmid # 8959 ; http://n2t.net/addgene:8959 ; RRID:Addgene_8959)
For your References section:Regulation of retinoblastoma protein functions by ectopic expression of human cyclins. Hinds PW, Mittnacht S, Dulic V, Arnold A, Reed SI, Weinberg RA. Cell. 1992 Sep 18. 70(6):993-1006. 10.1016/0092-8674(92)90249-C PubMed 1388095