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Filippo Del Bene Lab Plasmids


The Filippo Del Bene Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair. Auer TO, Duroure K, De Cian A, Concordet JP, Del Bene F Genome Res. 2014 Jan;24(1):142-53. doi: 10.1101/gr.161638.113. Epub 2013 Oct 31.
2C-Cas9: a versatile tool for clonal analysis of gene function. Di Donato V, De Santis F, Auer TO, Testa N, Sanchez-Iranzo H, Mercader N, Concordet JP, Del Bene F Genome Res. 2016 Mar 8.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
61051DR274-eGFP sgRNAEGFP sgRNA (Synthetic)CRISPRHighly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair. Genome Res. 2014 Jan;24(1):142-53. doi: 10.1101/gr.161638.113. Epub 2013 Oct 31. for generation of a eGFP specific sgRNA from a T7 promoter DR274 Add to Cart
61069eGFPbait-E2A-KalTA4-pA donor vectoreGFPbait (Synthetic), KalTA4 (Synthetic)zebrafish expressionHighly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair. Genome Res. 2014 Jan;24(1):142-53. doi: 10.1101/gr.161638.113. Epub 2013 Oct 31. for CRISPR/Cas9 mediated insertion of E2A-KalTA4; to be used in combination with a eGFP specific sgRNA (e.g. Plasmid 61051) pCRII-TOPO Add to Cart
74009pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA25x UAS (Saccharomyces cerevisiae), Cas9 (Other), T2A, GFP, U6 promoter (Danio rerio)CRISPR2C-Cas9: a versatile tool for clonal analysis of gene function. Genome Res. 2016 Mar 8. Tissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoter pminiTol2 Add to Cart
74010pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA25xUAS, Cas9, Cre, U6 promoterCRISPR2C-Cas9: a versatile tool for clonal analysis of gene function. Genome Res. 2016 Mar 8. Tissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoter pminiTol2 Add to Cart