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All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins.

Hochbaum DR, Zhao Y, Farhi SL, Klapoetke N, Werley CA, Kapoor V, Zou P, Kralj JM, Maclaurin D, Smedemark-Margulies N, Saulnier JL, Boulting GL, Straub C, Cho YK, Melkonian M, Wong GK, Harrison DJ, Murthy VN, Sabatini BL, Boyden ES, Campbell RE, Cohen AE
Nat Methods. 2014 Aug;11(8):825-33. doi: 10.1038/nmeth.3000. Epub 2014 Jun 22. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
51629DRH229: FCK-QuasAr1-mO2Fluorescent reporter for membrane voltage with improved brightness
51692DRH334: FCK-QuasAr2-mO2Fluorescent reporter of membrane voltage with improved sensitivity
51693DRH313: FCK-CheRiff-eGFPChannelrhodopsin with improved sensitivity and minimal red light photocurrent
51694DRH296: FCK-Optopatch2Coexpression plasmid of QuasAr2 and CheRiff for all-optical electrophysiology
51695DRH335: FCK-Optopatch1Coexpression plasmid of QuasAr1 and CheRiff for all-optical electrophysiology
51696DRH322: AAV-CaMKIIa-QuasAr2-mO2AAV production of QuasAr2 fluorescent reporter
51697DRH337: AAV-hsyn-CheRiff-eGFPAAV production of CheRiff channelrhodopsin actuator
62984pMOS001: CMV-Optopatch2_FCKCoexpression plasmid of QuasAr2 and CheRiff for all-optical electrophysiology under CMV promoter
62985pMOS009: pLX304-QuasAr2Lentivirus production of QuasAr2 fluorescent reporter
62986pMOS010: pLX304-CheRiff-GFPLentivirus production of CheRiff channelrhodopsin actuator
64134pBAD_His B_QuasAr2Fluorescent reporter of membrane voltage with improved sensitivity
64135pBAD_His B_QuasAr1Fluorescent reporter for membrane voltage with improved brightness

Antibodies from Article