DRH229: FCK-QuasAr1-mO2
(Plasmid #51629)

• Purpose: Fluorescent reporter for membrane voltage with improved brightness
• Depositing Lab: Adam Cohen
• Publication: Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi: 10.1038/nmeth.3000. Epub 2014 Jun 22.

Backbone

• Vector backbone: FCK(1.3)
• Backbone manufacturer: Pavel Osten
• Backbone size w/o insert (bp): 9349
• Total vector size (bp): 10799
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Use recombinase-free E. coli (e.g., Invitrogen's Stbl3)
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: QuasAr1-mOrange
• Alt name: Arch3.77H
• Species: Synthetic
• Insert Size (bp): 1560
• Promoter: CaMKIIa
• Tag / Fusion Protein:
  • mOrange2 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: gcctctttgccccacttaat
• 3′ sequencing primer: CATAGCGTAAAAGGAGCAACA

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry