This website uses cookies to ensure you get the best experience. By continuing the use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Dyche Mullins Lab Plasmids


The Dyche Mullins Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

Learn more about research in the Dyche Mullins Lab.

Addgene Alerts

You must login or create an account to subscribe for Addgene Alerts.

Title Authors Publication
DNA damage induces nuclear actin filament assembly by formin-2 and Spire-1/2 that promotes efficient DNA repair. Belin BJ, Lee T, Mullins RD Elife. 2015 Aug 19;4. doi: 10.7554/eLife.07735.
Comparative analysis of tools for live cell imaging of actin network architecture. Belin BJ, Goins LM, Mullins RD Bioarchitecture. 2015 Aug 28:1-14.
Visualization of actin filaments and monomers in somatic cell nuclei. Belin BJ, Cimini BA, Blackburn EH, Mullins RD Mol Biol Cell. 2013 Apr;24(7):982-94. doi: 10.1091/mbc.E12-09-0685. Epub 2013 Feb 27.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
58466pEGFP-C1 Utr230-EGFP-3XNLSUtr230 with 3X glycine linker (Homo sapiens), 3X NLS with 3X stop codons (Other)Mammalian ExpressionVisualization of actin filaments and monomers in somatic cell nuclei. Mol Biol Cell. 2013 Apr;24(7):982-94. doi: 10.1091/mbc.E12-09-0685. Epub 2013 Feb 27. Expresses a nuclear-targeted actin filament reporter, consisting of the first 230 amino acids of human utrophin, on a CMV promoter pEGFP-C1 Add to Cart
58467pEGFP-C1 Lifeact-EGFP-2XNLSLifeact (Synthetic), 2X NLS with 2X stop codons (Other)Mammalian ExpressionVisualization of actin filaments and monomers in somatic cell nuclei. Mol Biol Cell. 2013 Apr;24(7):982-94. doi: 10.1091/mbc.E12-09-0685. Epub 2013 Feb 27. Expresses a nuclear-targeted actin filament reporter, consisting of the peptide Lifeact, on a CMV promoter pEGFP-C1 Add to Cart
58468pEGFP-C1 EGFP-3XNLS3X NLS with 3X stop codons (Other)Mammalian ExpressionVisualization of actin filaments and monomers in somatic cell nuclei. Mol Biol Cell. 2013 Apr;24(7):982-94. doi: 10.1091/mbc.E12-09-0685. Epub 2013 Feb 27. Expresses a nuclear-targeted eGFP on a CMV promoter pEGFP-C1 Add to Cart
58469pEGFP-C1 RPEL1-EGFP-3XNLSMRTF-A RPEL1 (Mus musculus), 3X NLS with 3X stop codons (Other)Mammalian ExpressionVisualization of actin filaments and monomers in somatic cell nuclei. Mol Biol Cell. 2013 Apr;24(7):982-94. doi: 10.1091/mbc.E12-09-0685. Epub 2013 Feb 27. Expresses a nuclear-targeted monomeric actin reporter, consisting of the RPEL1 domain from MAL/MRTF, on a CMV promoter pEGFP-C1 Add to Cart
58470pEGFP-C1 Lifeact-EGFPLifeact (Synthetic)Mammalian ExpressionComparative analysis of tools for live cell imaging of actin network architecture. Bioarchitecture. 2015 Aug 28:1-14. Expresses a cytoplasmic actin filament reporter, the peptide Lifeact, on a CMV promoter pEGFP-C1 Add to Cart
58471pEGFP-C1 Utr261-EGFPUtr261 (Homo sapiens)Mammalian ExpressionComparative analysis of tools for live cell imaging of actin network architecture. Bioarchitecture. 2015 Aug 28:1-14. Expresses a cytoplasmic actin filament reporter, the tandem CH domains (a.a. 1-261) from human utrophin, on a CMV promoter pEGFP-C1 Add to Cart
58472pEGFP-C1 Utr230-EGFPUtr230 (Homo sapiens)Mammalian ExpressionComparative analysis of tools for live cell imaging of actin network architecture. Bioarchitecture. 2015 Aug 28:1-14. Expresses a cytoplasmic actin filament reporter, a truncation of the tandem CH domains (a.a. 1-230) from human utrophin, on a CMV promoter pEGFP-C1 Add to Cart
58473pEGFP-C1 F-tractin-EGFPF-tractin (Rattus norvegicus)Mammalian ExpressionComparative analysis of tools for live cell imaging of actin network architecture. Bioarchitecture. 2015 Aug 28:1-14. Expresses a cytoplasmic actin filament reporter, the neuronal inositol 1,4,5-triphosphate 3-kinase A actin-binding domain known as F-tractin, on a CMV promoter pEGFP-C1 Add to Cart
58475pmCherry-C1 actin-3XNLS P2A mCherrybeta actin (Homo sapiens), 3XNLS (Other), P2A cleavage site (Other)Mammalian ExpressionDNA damage induces nuclear actin filament assembly by formin-2 and Spire-1/2 that promotes efficient DNA repair. Elife. 2015 Aug 19;4. doi: 10.7554/eLife.07735. Expresses nuclear-targeted human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter mCherry-C1 Add to Cart
58476pmCherry-C1 mCherry-NLS3X NLS with 3X stop codons (Other)Mammalian ExpressionDNA damage induces nuclear actin filament assembly by formin-2 and Spire-1/2 that promotes efficient DNA repair. Elife. 2015 Aug 19;4. doi: 10.7554/eLife.07735. Expresses a nuclear-targeted mCherry control construct, on a CMV reporter pmCherry-C1 Add to Cart
58477pmCherry-C1 R62D actin-3XNLS P2A mCherrybeta actin (Homo sapiens), 3XNLS (Other), P2A cleavage site (Other)Mammalian ExpressionDNA damage induces nuclear actin filament assembly by formin-2 and Spire-1/2 that promotes efficient DNA repair. Elife. 2015 Aug 19;4. doi: 10.7554/eLife.07735. Expresses nuclear-targeted non-polymerizing R62D mutant of human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter mCherry-C1 Add to Cart