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pMKO.1 puro hTERT shRNA
(Plasmid #10688)


Item Catalog # Description Quantity Price (USD)
Plasmid 10688 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 6700
  • Vector type
    Mammalian Expression, Retroviral, RNAi
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    hTERT shRNA
  • Alt name
  • Alt name
    telomerase reverse transcriptase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Entrez Gene
    TERT (a.k.a. CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pLXSN 5'
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

hTERT 3114-3134 (coding region): Forward TTTCATCAGCAAGTTTGGAttcaagagaTCCAAACTTGCTGATGAAAtttttg ; Reverse aattcaaaaaTTTCATCAGCAAGTTTGGAtctcttgaaTCCAAACTTGCTGATGAAA .

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMKO.1 puro hTERT shRNA was a gift from William Hahn (Addgene plasmid # 10688 ; ; RRID:Addgene_10688)
  • For your References section:

    The telomerase reverse transcriptase regulates chromatin state and DNA damage responses. Masutomi K, Possemato R, Wong JM, Currier JL, Tothova Z, Manola JB, Ganesan S, Lansdorp PM, Collins K, Hahn WC. Proc Natl Acad Sci U S A. 2005 Jun 7. 102(23):8222-7. 10.1073/pnas.0503095102 PubMed 15928077