pMKO.1 puro GFP shRNA
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||10675||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6700
Vector typeMammalian Expression, Retroviral, RNAi
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameGFP shRNA
Insert Size (bp)58
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (destroyed during cloning)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pLXSN 5' (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
shRNA oligo sequence: 5'-GCTACGTCCAGGAGCGCACCCTCGAGGGTGCGCTCCTGGACGTAGCC
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMKO.1 puro GFP shRNA was a gift from William Hahn (Addgene plasmid # 10675 ; http://n2t.net/addgene:10675 ; RRID:Addgene_10675)
For your References section:The telomerase reverse transcriptase regulates chromatin state and DNA damage responses. Masutomi K, Possemato R, Wong JM, Currier JL, Tothova Z, Manola JB, Ganesan S, Lansdorp PM, Collins K, Hahn WC. Proc Natl Acad Sci U S A. 2005 Jun 7. 102(23):8222-7. 10.1073/pnas.0503095102 PubMed 15928077