Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pMIEG3-JunDN
(Plasmid #40350)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 40350 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMIEG3
  • Backbone manufacturer
    David A. Williams (PMID: 10961859)
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Jun dominant negative
  • Alt name
    JunDN
  • Alt name
    c-Jun
  • Species
    H. sapiens (human)
  • Mutation
    first 122 amino acids deleted
  • Entrez Gene
    JUN (a.k.a. AP-1, AP1, c-Jun, cJUN, p39)
  • Tags / Fusion Proteins
    • HA (C terminal on insert)
    • IRES eGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pLXSN_5
  • 3′ sequencing primer IRES-R2 (gacggcaatatggtggaaa)
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The Jun dominant negative vector was made by first preparing a version of c-Jun with the first 122 aa deleted. This cDNA was amplified by PCR from human c-Jun with the following primers:

sense, 5-AAAAAA-GAATTC-ATGACTAGCCAGAACACGCTGCCCAGCGTC-3;

antisense, 5-AAAAAA-GAATTC-TCAGCTGGCATAGTCAGGCACGTCATAAGGATAGCTAAATGTTTGCAAC-3.

The antisense primer adds a hemagglutinin tag to the C terminus of c-Jun. The PCR product was then cut with EcoRI and cloned into the retroviral vector pMIEG3.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMIEG3-JunDN was a gift from Alexander Dent (Addgene plasmid # 40350 ; http://n2t.net/addgene:40350 ; RRID:Addgene_40350)
  • For your References section:

    Regulation of IL-10 gene expression in Th2 cells by Jun proteins. Wang ZY, Sato H, Kusam S, Sehra S, Toney LM, Dent AL. J Immunol. 2005 Feb 15;174(4):2098-105. PubMed 15699140