|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||33353||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5500
Vector typeMammalian Expression
Growth in Bacteria
SpeciesM. musculus (mouse)
MutationDominant Negative of c-Jun; heterodimerizes and inhibits c-Jun
Entrez GeneFos (a.k.a. D12Rfj1, c-fos, cFos)
- Promoter CMV
/ Fusion Protein
- FLAG (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer CMV-Fwd
- 3′ sequencing primer BGH-Rev (Common Sequencing Primers)
Acidic amphipathic extension added to the Fos leucine zipper: LEQRAEELARENEELEKEAEELEQELAE
Although the acidic extension is to the c-Fos leucine zipper, A-Fos heterodimerizes and inhibits c-Jun.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CMV500 A-FOS was a gift from Charles Vinson (Addgene plasmid # 33353 ; http://n2t.net/addgene:33353 ; RRID:Addgene_33353)
For your References section:A dominant-negative inhibitor of CREB reveals that it is a general mediator of stimulus-dependent transcription of c-fos. Ahn S, Olive M, Aggarwal S, Krylov D, Ginty DD, Vinson C. Mol Cell Biol. 1998 Feb . 18(2):967-77. PubMed 9447994