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CMV500 empty vector
(Plasmid #33348)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 33348 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size (bp) 5500
  • Vector type
    Mammalian Expression
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

The polylinker region of the pRc/CMV eukaryotic expression vector (Invitrogen) was cut with HindIII and XbaI, and the following annealed oligonucleotide was inserted: 5′-AGCTCCACCATGGACTACAAGGACGACGATGACAAGCATATGTGATGAAGCTT-3′ (underlined bases are restriction sites for NcoI, NdeI, and HindIII) and 5′-CTAGAAGCTTCATCACATATGCTTGTCATCGTCGTCCTTGTAGTCCATGGTGG-3′ (underlined bases are restriction site for XbaI). The HindIII restriction site was not regenerated and was reintroduced downstream of the FLAG sequence (DYKDDDDK).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV500 empty vector was a gift from Charles Vinson (Addgene plasmid # 33348 ; ; RRID:Addgene_33348)
  • For your References section:

    SREBP-1 dimerization specificity maps to both the helix-loop-helix and leucine zipper domains: use of a dominant negative. Rishi V, Gal J, Krylov D, Fridriksson J, Boysen MS, Mandrup S, Vinson C. J Biol Chem. 2004 Mar 19;279(12):11863-74. Epub 2003 Dec 31. 10.1074/jbc.M308000200 PubMed 14702347