|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||40348||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerDavid A. Williams (PMID: 10961859)
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Alt namejun proto-oncogene
SpeciesH. sapiens (human)
GenBank IDNM_002228.3 NP_002219.1
Entrez GeneJUN (a.k.a. AP-1, AP1, c-Jun, cJUN, p39)
/ Fusion Protein
- IRES eGFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer pLXSN_5
- 3′ sequencing primer IRES-R2 (gacggcaatatggtggaaa) (Common Sequencing Primers)
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The c-Jun- and JunB-expressing retroviruses were constructed by inserting c-Jun and JunB cDNAs into the retroviral vector pMIEG3 via NotI and XhoI sites.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMIEG3-c-Jun was a gift from Alexander Dent (Addgene plasmid # 40348 ; http://n2t.net/addgene:40348 ; RRID:Addgene_40348)
For your References section:Regulation of IL-10 gene expression in Th2 cells by Jun proteins. Wang ZY, Sato H, Kusam S, Sehra S, Toney LM, Dent AL. J Immunol. 2005 Feb 15;174(4):2098-105. PubMed 15699140