Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #40348)


Item Catalog # Description Quantity Price (USD)
Plasmid 40348 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    David A. Williams (PMID: 10961859)
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    jun proto-oncogene
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_002228.3 NP_002219.1
  • Entrez Gene
    JUN (a.k.a. AP-1, AP1, c-Jun, cJUN, p39)
  • Tag / Fusion Protein
    • IRES eGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer pLXSN_5
  • 3′ sequencing primer IRES-R2 (gacggcaatatggtggaaa)
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The c-Jun- and JunB-expressing retroviruses were constructed by inserting c-Jun and JunB cDNAs into the retroviral vector pMIEG3 via NotI and XhoI sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMIEG3-c-Jun was a gift from Alexander Dent (Addgene plasmid # 40348 ; ; RRID:Addgene_40348)
  • For your References section:

    Regulation of IL-10 gene expression in Th2 cells by Jun proteins. Wang ZY, Sato H, Kusam S, Sehra S, Toney LM, Dent AL. J Immunol. 2005 Feb 15;174(4):2098-105. PubMed 15699140