Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 4,700 results for: golden gate

Clear keywords
Showing: 3341 - 3360 of 4700 results

  13. pShHELIX_sgRNA_entry (CJT32)

    Plasmid
    #181782
    Purpose
    Expresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181782-plasmid-map-sequence-id-354808

    Enlarge

  15. pC-GoldyTALEN

    Plasmid
    #38143
    Purpose
    destination vector for the Golden Gate TALEN kit, directs expression of TALENs from a truncated CAGs promoter
    Depositor
    Daniel Carlson
    Article
    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.
    Insert
    GoldyTALEN
    Tags
    AcV5 and FokI homodimer
    Expression
    Mammalian
    Mutation
    Truncate at N and C terminous
    Promoter
    miniCaggs
    Available Since
    July 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    38143-plasmid-map-sequence-id-48254

    Enlarge

  17. pAcHELIX-sgRNA_entry (CJT94)

    Plasmid
    #181783
    Purpose
    Expresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181783-plasmid-map-sequence-id-358223

    Enlarge

Showing: 3341 - 3360 of 4700 results