We narrowed to 18,530 results for: pET

1. [pSK122] pETDuet GST-p18(delta5)

   Plasmid

   #136144

   Purpose

   bacterial expression of GST-p18

   Depositor

   David Sabatini, Kuang Shen

   Article

   Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

   Insert

   GST-p18
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21a(+)-HIV RT-6xHis-RBS-prot

   Plasmid

   #159149

   Purpose

   expresses His-tagged HIV reverse transcriptase and untagged HIV protease

   Depositor

   Andrea Pauli

   Article

   Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. d

   Inserts

   Human immunodeficiency virus 1 (HIV-1) reverse transcriptase
   Human immunodeficiency virus 1 (HIV-1) protease
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   HIV-1 group M/subtype B – BH10 strain

   Promoter

   Available since

   Sept. 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pET51b-His-TEV-CLIP-tag

   Plasmid

   #167274

   Purpose

   Overexpression of CLIP-tag in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   CLIP-tag
   

   Use

   Tags

   His x10

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET 28 full-length human PARP1

   Plasmid

   #192635

   Purpose

   Expresses human PARP1 with an N-terminal His-tag

   Depositor

   John Pascal

   Article

   Langelier et al J Biol Chem. 2008 Feb 15;283(7):4105-14.

   Insert

   Human PARP1 (PARP1 Human)
   

   Use

   Tags

   histidine-tag

   Expression

   Bacterial

   Mutation

   Polymorphism A762

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. PET-28a-YTH domain of YTHDF1

   Plasmid

   #228775

   Purpose

   Protein expression plasmid for YTH domain of YTHDF1 in E.Coli.

   Depositor

   Huiqing Zhou

   Article

   Wang et al RSC Chem Biol. 2024 Jul 25;5(9):914-923.

   Insert

   YTH domain of YTHDF1 (YTHDF1 Human)
   

   Use

   Tags

   6*His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-GGG-ELP(40nm)-Cys

   Plasmid

   #91571

   Purpose

   Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

   Depositor

   Hermann Gaub

   Article

   Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

   Insert

   GGG-ELP(40nm)-Cys
   

   Use

   Tags

   Cysteine and Sortase-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-21a(+)-BMTU UDG-6xHis

   Plasmid

   #172197

   Purpose

   expresses His-tagged uracil-DNA-glycosylase enzyme from marine bacterium BMTU 3346 for the purpose of cross contamination prevention in nucleic acid amplification reactions

   Depositor

   Andrea Pauli

   Insert

   BMTU UDG
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. PET-28a-YTH domain of YTHDF3

   Plasmid

   #228777

   Purpose

   Protein expression plasmid for YTH domain of YTHDF3 in E.Coli.

   Depositor

   Huiqing Zhou

   Article

   Wang et al RSC Chem Biol. 2024 Jul 25;5(9):914-923.

   Insert

   YTH domain of YTHDF3 (YTHDF3 Human)
   

   Use

   Tags

   6*His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-28a RUVBL2 Full Length

   Plasmid

   #159142

   Purpose

   Expresssion and protein purification of full length RUVBL2

   Depositor

   Eric Erquan Zhang

   Article

   Ju et al Sci Transl Med. 2020 May 6;12(542). pii:

   Insert

   RUVBL2 (RUVBL2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pETDuet-1_6xHis-TEV-mCh-NDP52

    Plasmid

    #187829

    Purpose

    Plasmid for the expression and purification of 6xHis-TEV-mCh-NDP52 in E. coli Rosetta pLysS. Internal Reference: SMC1148

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    NDP52 (CALCOCO2 Human)
    

    Use

    Tags

    6xHisTag, TEV, and mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21-pelB-LaG-16-4GS-LaG-2

    Plasmid

    #172764

    Purpose

    Bacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (4GS linker)

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-16-4GS-LaG-2
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-30-His6-SUMO-AEY-CfaN

    Plasmid

    #122949

    Purpose

    Bacterial expression of N-intein

    Depositor

    Thomas Muir

    Article

    Stevens et al J Am Chem Soc. 2016 Feb 24;138(7):2162-5

    Insert

    CfaN
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pet151D-TOPO ALIX Bro1-V (1-702)

    Plasmid

    #80642

    Purpose

    Residues 1-702 of ALIX; Bacterial Expression Vector; TEV cleavable N-term His-tag; Sundquist Lab Internal ID: WISP10-648

    Depositor

    Wesley Sundquist

    Article

    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

    Insert

    ALIX Bro1-V (PDCD6IP Human)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Residues 1-702

    Promoter

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-CohIII-CBM-HIS-ybbR

    Plasmid

    #60866

    Purpose

    Characteristic fingerprint molecule fused to cohesin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced ybbR tag

    Depositor

    Hermann Gaub

    Article

    Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1

    Inserts

    CohIII
    CBM
    

    Use

    Tags

    6xHIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Mutation

    Cysteine 63 to Serine in CBM module

    Promoter

    T7

    Available since

    Nov. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-28-Strep-OPA1(MGD) (SB264)

    Plasmid

    #227613

    Purpose

    Inducible expression of Strep-tagged Opa1(MGD) in E.coli

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    OPA1 (OPA1 Human)
    

    Use

    Tags

    Twin-StrepTag

    Expression

    Bacterial

    Mutation

    contains aa 253-580 (MGD) and aa 938-960

    Promoter

    T7

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-HIS-Xyl-Xmod-DocIII

    Plasmid

    #60865

    Purpose

    Characteristic fingerprint molecule fused to dockerin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced Cystein in Xylanase T120C

    Depositor

    Hermann Gaub

    Article

    Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1

    Inserts

    Xyl
    Xmod-DocIII
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Threonine 120 to Cysteine in Xylanase domain

    Promoter

    T7

    Available since

    Nov. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET51b-His-TEV-CLIP-tag_fast

    Plasmid

    #167275

    Purpose

    Overexpression of the fast variant (E30R) of CLIP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    CLIPf-tag
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    E30R

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-29b-3xGluGlu-c-jun-His6

    Plasmid

    #129227

    Purpose

    Encodes 3xGluGlu tagged human c-Jun for purification by nickel affinity

    Depositor

    Kevin Janes

    Article

    Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

    Insert

    cJun (JUN Human)
    

    Use

    Tags

    6xHistidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET21-E. coli Initiation Factor 3 (IF3)

    Plasmid

    #31340

    Depositor

    Linda Spremulli

    Article

    Koc et al J Biol Chem. 2002 Sep 20. 277(38):35541-

    Insert

    E. coli Initiation Factor 3
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pET-Duet1_6xHis-TEV-eGFP-P62

    Plasmid

    #190929

    Purpose

    Plasmid for the expression and purification of GFP labelled p62. Internal reference: SMC390

    Depositor

    Sascha Martens

    Article

    Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

    Insert

    P62 (SQSTM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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