We narrowed to 15,158 results for: pET

1. pET12a-AL-103 Y32F Y96F

   Plasmid

   #47092

   Depositor

   Marina Ramirez-Alvarado

   Article

   DiCostanzo et al J Biol Chem. 2012 Aug 10;287(33):27997-8

   Insert

   AL-103 Y32F Y96F (IGKV3-20 Human)
   

   Tags

   ompT

   Expression

   Bacterial

   Mutation

   Y32F Y96F

   Promoter

   T7

   Available Since

   Aug. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pET21b-Tipin-His-L195A

   Plasmid

   #23123

   Depositor

   Aziz Sancar

   Article

   Kemp et al J Biol Chem. 2010 May 28;285(22):16562-7

   Insert

   Tipin (TIPIN Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Changed Leucine 195 to Alanine

   Available Since

   Jan. 28, 2010

   Availability

   Academic Institutions and Nonprofits only

3. HI0058/pET-21a(+)c-tag

   Plasmid

   #13097

   Depositor

   SeWon Suh

   Article

   Ku et al Acta Crystallogr D Biol Crystallogr. 200

   Insert

   3-deoxy-manno-octulosonate cytidylyltransferase (kdsB Haemophilus influenzae)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   June 29, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-21a(+) SurE D8A

   Plasmid

   #12685

   Depositor

   SeWon Suh

   Article

   Lee et al Nat Struct Biol. 2001 Sep . 8(9):789-94.

   Insert

   SurE (TM1662 )
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   changed Aspartate 8 to Alanine

   Available Since

   Jan. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pET His10-Draper INT

   Plasmid

   #126670

   Purpose

   Expression of His10-Draper intracellular domain for purification from E. coli

   Depositor

   Ron Vale

   Article

   Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

   Insert

   Draper cytoplasmic domain
   

   Expression

   Bacterial

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-PKAsub(T/A)-YPet-NES

   Plasmid

   #138205

   Purpose

   Encodes negative-control substrate fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.

   Depositor

   Jin Zhang

   Article

   Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

   Insert

   PKAsub(T/A)-YPet-NES
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-PKAsub-YPet-Kras

   Plasmid

   #138206

   Purpose

   Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (bimAKAR); plasma membrane targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.

   Depositor

   Jin Zhang

   Article

   Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

   Insert

   PKAsub-YPet-Kras
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Availability

   Academic Institutions and Nonprofits only

   

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8. Pet28a His6-HSPB7 (zebrafish) WT

   Plasmid

   #108917

   Purpose

   Express WT Zebrafish His6-HSPB7 in bacteria

   Depositor

   Yimon Aye

   Article

   Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

   Insert

   His6-HSPB7 (hspb7 Zebrafish)
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-SdrG_N2N3-B1-B2-HIS-ybbr

   Plasmid

   #117979

   Purpose

   SdrG N2 and N3, B1 and B2 domains with C-terminal ybbr tag for covalent immobilization

   Depositor

   Hermann Gaub

   Article

   Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

   Insert

   SdrG_N2N3-B1-B2
   

   Tags

   H3V 3C, HIS, and ybbr

   Expression

   Bacterial

   Promoter

   T7

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-SdrG_N2N3-B1-HIS-ybbr

    Plasmid

    #117980

    Purpose

    SdrG N2 and N3, B1 domains with C-terminal ybbr tag for covalent immobilization

    Depositor

    Hermann Gaub

    Article

    Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

    Insert

    SdrG_N2N3-B1
    

    Tags

    H3V 3C, HIS, and ybbr

    Expression

    Bacterial

    Promoter

    T7

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28-MBP-super TEV protease

    Plasmid

    #171782

    Purpose

    Expresses Maltose-binding protein-super TEV protease in bacterial cytoplasm. This protease version performs well in the absence of added reducing agent.

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    MBP-sTEV
    

    Tags

    Arg6 tag and Internal His6 tag

    Expression

    Bacterial

    Mutation

    No internal TEV cleavage site between the MBP and…

    Promoter

    T7

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. PET-HiFi SpCas9-NLS-6xHis

    Plasmid

    #207376

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    HiFi SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R691A

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET21a asyn(Δ41-54/Δ103-130)

    Plasmid

    #242236

    Purpose

    Expresses alpha synuclein (missing exon 3 and 5, residues 41-54 and 103-130) in bacteria

    Depositor

    Jennifer Lee

    Article

    SanGiovanni et al Protein Sci. 2025 Jul;34(7):e70195. doi:

    Insert

    asyn(Δ41-54/Δ103-130)
    

    Tags

    none

    Expression

    Bacterial

    Mutation

    deleted amino acids 41-54 and 103-130

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETDuet1_6xHis-TEV-Lambda-Protein-Phosphatase

    Plasmid

    #223747

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    Lambda-PPase
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-MCN-10His-mStayGold (E138D)

    Plasmid

    #211361

    Purpose

    Bacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

    Depositor

    Mohan Balasubramanian

    Article

    Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

    Insert

    mStayGold
    

    Tags

    10xHis-tag

    Expression

    Bacterial

    Mutation

    E138D

    Promoter

    T7

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21a(+)-HIV RT-6xHis-RBS-prot

    Plasmid

    #159149

    Purpose

    expresses His-tagged HIV reverse transcriptase and untagged HIV protease

    Depositor

    Andrea Pauli

    Article

    Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. d

    Inserts

    Human immunodeficiency virus 1 (HIV-1) reverse transcriptase
    Human immunodeficiency virus 1 (HIV-1) protease
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    HIV-1 group M/subtype B – BH10 strain

    Available Since

    Sept. 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pET29-bdSUMO-Anti-ALFA nanobody

    Plasmid

    #212311

    Purpose

    Expression of anti-ALFA nanobody

    Depositor

    Arthur Laganowsky

    Article

    Stover et al J Struct Biol X. 2024 Feb 2;9:100097. do

    Insert

    anti-ALFA nanobody
    

    Tags

    bdSUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-28c-F30-2xdBroccoli-47CAG

    Plasmid

    #237217

    Purpose

    Express F30-2xdBroccoli tagged 47 repeats of CAG RNA in bacteria.

    Depositor

    Mingxu You

    Article

    Xue et al Nucleic Acids Res. 2023 Sep 8;51(16):833

    Insert

    47 CAG trinucleotide repeats RNA
    

    Expression

    Bacterial

    Available Since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-30a-NSP14-SARS-CoV-2

    Plasmid

    #236423

    Purpose

    bacterial protein expression of NSP14

    Depositor

    Michele Pagano

    Article

    Rona et al Cell Death Differ. 2022 Feb;29(2):285-29

    Insert

    NSP14 (ORF1ab SARS-CoV-2)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET 28 full-length human PARP1

    Plasmid

    #192635

    Purpose

    Expresses human PARP1 with an N-terminal His-tag

    Depositor

    John Pascal

    Article

    Langelier et al J Biol Chem. 2008 Feb 15;283(7):4105-14.

    Insert

    Human PARP1 (PARP1 Human)
    

    Tags

    histidine-tag

    Expression

    Bacterial

    Mutation

    Polymorphism A762

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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