We narrowed to 5,185 results for: golden gate
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Plasmid#251996PurposeExpresses double-stranded RNA targeting the Def1 gene from Apis mellifera.DepositorInsertFragment of Defensin 1 gene
ExpressionInsectPromoterCP25 (no RBS)Available SinceMarch 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
AraC-S170-LOV-C450A-K488N(M393)
Plasmid#250777PurposeExpresses AraC-S170-LOV from a Trc promoter. For use in E. coliDepositorInsertAraC with an AsLOV2 insertion behind S170 and C450A and K488N mutation
UseSynthetic BiologyTagsHis-tagExpressionBacterialMutationC450A, K488NAvailable SinceMarch 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
AraC-S170-LOV-C450A-D432V(M392)
Plasmid#250776PurposeExpresses AraC-S170-LOV from a Trc promoter. For use in E. coliDepositorInsertAraC with an AsLOV2 insertion behind S170 and C450A and D432V mutation
UseSynthetic BiologyTagsHis-tagExpressionBacterialMutationC450A, D432VAvailable SinceMarch 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pBTK1210 (pDS-BiFC6)
Plasmid#251991PurposeDouble-stranded RNA biomolecular fluorescence complementation sensor for bacteria, induced by vanillic acid.DepositorInsertSplit mVenus (YFP) fused to mutant B2 dsRNA-binding domains (RBDs)
UseBiosensorExpressionBacterialPromoterPvanAvailable SinceFeb. 25, 2026AvailabilityAcademic Institutions and Nonprofits only -
pBTK1212
Plasmid#251993PurposeDouble-stranded RNA biomolecular fluorescence complementation sensor for bacteria, induced by arabinose. Variant of pDS-BiFC7 with AmpR.DepositorInsertSplit mVenus (YFP) fused to mutant B2 dsRNA-binding domains (RBDs)
UseBiosensorExpressionBacterialPromoterPBADAvailable SinceFeb. 25, 2026AvailabilityAcademic Institutions and Nonprofits only -
pSHARK6
Plasmid#248169PurposepBBR1 plasmid with constituitive lambda-CI (J23101-Bujard-Lambda-CI) and PirWT (PTet-RJ64-Pir4150) expressionDepositorInsertLambdaCI-Pir4150
ExpressionBacterialMutationPir: T108I and P113SAvailable SinceFeb. 23, 2026AvailabilityAcademic Institutions and Nonprofits only -
pR6K-mScarI
Plasmid#248166PurposeR6K plasmid with constituitive red fluorescent protein (mScarlet-I) expression under J23101 promoterDepositorInsertmScarlet-I
ExpressionBacterialMutationNoneAvailable SinceFeb. 19, 2026AvailabilityAcademic Institutions and Nonprofits only -
BCTVΔVS-RT-pDGB3alpha2
Plasmid#240730PurposeBCTV vector containing the GUS:NPTII repari template. Lacks the BCTV virion sense ORFs.DepositorInsertRT
ExpressionPlantMutation2 stop codons inserted in RepAvailable SinceFeb. 10, 2026AvailabilityAcademic Institutions and Nonprofits only -
pHREP-KD-1-shH2B
Plasmid#240417PurposeSleeping Beauty vector for inducible expression of a chicken H2B targeting shRNA using a shortened mir30 sequence. Allows co-selection with Puromycin.DepositorInsertH2B
ExpressionMammalianPromoterTREAvailable SinceFeb. 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
pSBinit-Syb-BC10(Y35A/Y56A/R60A/I61T/H63A/T105A/Y107A/W108S)
Plasmid#250415PurposeExpression of Anti-SLC66A2 Sybody Non-Binding Variant in BacteriaDepositorInsertSyb-BC10
UseAffinity Reagent/ AntibodyTagsMyc-6xHis and PelBExpressionBacterialMutationCDR1(Y35A)/CDR2(Y56A/R60A/I61T/H63A)/CDR3(T105A/Y…PromoteraraBADAvailable SinceFeb. 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
pEM547
Plasmid#248742PurposePBT0779 biosensor circuit (PTetR56 version) in pNBU2 integrative Bacteroides plasmidDepositorInsertPBfP1E6-RBS1PLP-mScarletI3-rrnBT2-PBT0779-TetR-rrnBT2-pTetR56-RBS1PLP-mGL-rrnBT2
ExpressionBacterialAvailable SinceFeb. 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
BeYDV-RT-pUPD2
Plasmid#240747PurposeBeYDV viral vector containing a repair template.DepositorInsertBeYDV:RT
UseSynthetic BiologyAvailable SinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
BCTV-VS domesticated-pUPD2
Plasmid#240748PurposeDomesticated BCTV virion sense ORFs with A1-B1 Goldenbraid grammarDepositorInsertBCTV VS
UseSynthetic BiologyMutation2nt substitution in V3Available SinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
BCTV-CS-pUPD2
Plasmid#240746PurposeBCTV complementary sense ORFs with C1 Goldenbraid grammarDepositorInsertBCTV CS
UseSynthetic BiologyAvailable SinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
BCTVΔREP-pUPD2
Plasmid#240715PurposeBCTV complementary sense ORFs with mutated Rep with GoldenBraid C1 grammarDepositorInsertBCTV CS
UseSynthetic BiologyMutation2 stop codons inserted in RepAvailable SinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
BCTV-VS WT-pUPD2
Plasmid#240716PurposeWT BCTV virion sense ORFs prior to domestication for GoldenGate cloning with A1-B1 Goldenbraid grammarDepositorInsertBCTV VS
UseSynthetic BiologyAvailable SinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
BCTVΔIR-pUPD2
Plasmid#240717PurposeBCTV complementary sense ORFs lacking the IR. Has C1 Goldenbraid grammar.DepositorInsertBCTV CS
UseSynthetic BiologyAvailable SinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
BCTVΔVS-pUPD2
Plasmid#240718PurposeBCTV IR lacking virion sense ORFs.DepositorInsertBCTV IR
UseSynthetic BiologyAvailable SinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
RT B2-B6-pUPD2
Plasmid#240719PurposeGUS:NPTII repair template used for HDR experiments with compatible reporter lines. Has B2-B6 Goldenbraid grammar.DepositorInsertRT
UseSynthetic BiologyAvailable SinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
RT B6-pUPD2
Plasmid#240720PurposeGUS:NPTII repair template used for HDR experiments with compatible reporter lines. Has B6 Goldenbraid grammar.DepositorInsertRT
UseSynthetic BiologyAvailable SinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only