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Mouse lipid metabolism knockout library

Pooled library

#206248

Purpose

The lipid metabolism library contains guide RNAs against 295 genes targeting mouse genes encoding enzymes that directly impact lipid metabolism.

Depositor

Kivanc Birsoy

Publication

Soula et al Nature. 2024 Aug 7. doi: 10.1038/s41586-

Use

CRISPR and Lentiviral

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only
AiP12542: pAAV-AiE0410m-minBG-mTFP1-WPRE3-BGHpA (Alias: CN2542)

Plasmid

#236603

Purpose

AiE0410m is an enhancer sequence, designed to drive AAV-mediated transgene expression in oligodendrocytes

Depositor

Jonathan Ting

Publication

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

mTFP1

Use

AAV

Available since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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AiP15385: pAAV-AiE0873m_3xC2-minBG-mScarlet-WPRE3-BGHpA (Alias: CN5385)

Plasmid

#230875

Purpose

AiE0873m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal cholinergic interneurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

mScarlet

Use

AAV

Expression

Mammalian

Available since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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AiP15100: pAAV-AiE0140h_3xC2-minBG-FlpO-4X2C-WPRE3-BGHpA (Alias: CN5100)

Plasmid

#230873

Purpose

AiE0140h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical and striatal Pvalb interneurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

FlpO

Use

AAV

Expression

Mammalian

Available since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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AiP14646: pAAV-AiE0779m_6xC2-minBG-jGCaMP8s-WPRE3-BGHpA (Alias: CN4646)

Plasmid

#230872

Purpose

AiE0779m_6xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal direct pathway MSNs (D1-MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

jGCaMP8s

Use

AAV

Expression

Mammalian

Available since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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AiP14647 - pAAV-AiE0452h-minBG-jGCaMP8s-WPRE3-BGHpA (Alias: CN4647)

Plasmid

#224185

Purpose

AiE0452h is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal D2 MSNs

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

jGCaMP8s

Use

AAV

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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AiP15828 - pAAV-AiE0780m-HSP68-SYFP2-WPRE3-BGHpA (Alias: CN5828)

Plasmid

#224207

Purpose

AiE0780m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal D1 MSNs

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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AiP14202 - pAAV-AiE0743m_3xC2-minBG-mScarlet-WPRE3-BGHpA (Alias: CN4202)

Plasmid

#224178

Purpose

AiE0743m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal cholinergic neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

mScarlet

Use

AAV

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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AiP12447 - pAAV-AiE0617h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2447)

Plasmid

#224164

Purpose

AiE0617h is an enhancer sequence, designed to drive AAV-mediated transgene expression in D2 MSN subtypes (note Olf bulb)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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AiP12448 - pAAV-AiE0618h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2448)

Plasmid

#224165

Purpose

AiE0618h is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal MSNs (dSTR>vSTR)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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AiP13808 - pAAV-AiE0140h_3xC2-minBG-mTFP1-WPRE3-BGHpA (Alias: CN3808)

Plasmid

#224176

Purpose

AiE0140h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal Pvalb+ neurons (note cortical Pvalb+ and L5_ET)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

mTFP1

Use

AAV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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AiP15143 - pAAV-AiE0452h-minBG-SYFP2-P2A-mScarlet-10aa-H2B-WPRE3-BGHpA (Alias: CN5143)

Plasmid

#224193

Purpose

AiE0452h is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal D2 MSNs

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

mScarlet-10aa-H2B

Use

AAV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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AiP15508 - pAAV-AiE1419m-minBG-iCre(R297T)-BGHpA (Alias: CN5508)

Plasmid

#224199

Purpose

AiE1419m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal Pvalb+/Pthlh+ neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

iCre(R297T)

Use

AAV

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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AiP15050 - pAAV-AiE1426m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN5050)

Plasmid

#224192

Purpose

AiE1426m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal Sst_Chodl neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2-P2A-3XFLAG-10aa-H2B

Use

AAV

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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AiP15041 - pAAV-AiE1417m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN5041)

Plasmid

#224187

Purpose

AiE1417m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal Pvalb+/Pthlh+ neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2-P2A-3XFLAG-10aa-H2B

Use

AAV

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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AiP14827: pAAV-AiE0441h_3xC2-minBG-jGCaMP8s-WPRE3-BGHpA (Alias: CN4827)

Plasmid

#214864

Purpose

Expression of jGCaMP8s in striatal medium spiny neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

jGCaMP8s

Use

AAV

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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AiP14871: pAAV-AiE0779m_3xC2-minBG-RiboL1-jGCaMP8s-WPRE3-BGHpA (Alias: CN4871)

Plasmid

#214865

Purpose

Expression of RiboL1-jGCaMP8s (soma targeted) in striatal direct pathway medium spiny neurons (D1 MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Has service

AAV1

Insert

RiboL1-jGCaMP8s

Use

AAV

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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AiP14948: pAAV-AiE0441h_3xC2-minBG-DIO-SYFP2-WPRE3-BGHpA (Alias: CN4948)

Plasmid

#214867

Purpose

Expression of SYFP2 in striatal medium spiny neurons in a Cre dependent manner; useful for intersectional labeling

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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AiP15148: pAAV-AiE0779m_6xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN5148)

Plasmid

#214870

Purpose

Expression of CoChR-EGFP in striatal direct pathway medium spiny neurons (D1 MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

CoChR-EGFP

Use

AAV

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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AiP14823: pAAV-AiE0441h_3xC2-minBG-ChR2(H134R)-EYFP-WPRE3-BGHpA (Alias: CN4823)

Plasmid

#214860

Purpose

Expression of ChR2(H134R)-EYFP in striatal medium spiny neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

ChR2(H134R)-EYFP

Use

AAV

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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