We narrowed to 5,025 results for: golden gate

Showing: 3701 - 3720 of 5025 results

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pKL1-423

Plasmid

#219411

Purpose

Reporter gene (2 operator) for zinc finger activator

Depositor

Ahmad Khalil

Article

Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

Insert

(42-10 op)x2-minCyc1-Venus-tCyc1

Expression

Yeast

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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L0-I26 pUBQ10 GG0-GG1

Plasmid

#195902

Purpose

Level 0 pUBQ10 promoter with GG0 and GG1 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pUBQ10 (UBQ10 Mustard Weed)

Use

Synthetic Biology

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pMOD_C'4800EC

Plasmid

#176875

Purpose

Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTSB-FICAT_Payload-Esp3I

Plasmid

#179380

Purpose

Transposon vector prepared for Esp3I golden gate cloning of Transgene

Depositor

Marc Güell

Article

Pallares-Masmitja et al Nat Commun. 2021 Dec 3;12(1):7071. doi:

Type

Empty backbone

Expression

Mammalian

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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CSM160

Plasmid

#105684

Purpose

Plasmid containing an RFP dropout used to construct randomized PAM library

Depositor

Chase Beisel

Article

Maxwell et al Methods. 2018 Jul 1;143:48-57. doi: 10.1

Insert

RFP Golden Gate dropout

Expression

Bacterial

Promoter

Bba_R0040 TetR Promoter

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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Sth_I-E array_GFP dropout

Plasmid

#178775

Purpose

Golden Gate GFP dropout vector to generate S. thermophilus type I-E single arrays

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

gfp dropout to generate S. thermophilus type I-E single arrays

Expression

Bacterial

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pJo55

Plasmid

#159623

Purpose

An empty HIS3 plasmid for translation factor eIF4E complementation test in yeast, strain Jo55. Golden Gate cloning using BsaI. Selection with RFP after cloning.

Depositor

Vasiliy Taranov

Article

Lebedeva et al Biochemistry (Mosc). 2021 Sep;86(9):1128

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pL0-BvCYP76AD1

Plasmid

#162529

Purpose

Beta vulgaris cytochrome P450 76AD1 coding sequence in pICH41308 MoClo Golden Gate level 0 acceptor for CDS1 modules.

Depositor

Sam Brockington

Article

Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

Insert

CYP76AD1

Use

Puc19-derived

Expression

Bacterial

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pJM457 EF1alpha EBFP2-P2A-BlastR (TUPV8)

Plasmid

#139237

Purpose

mMoClo TUPV8, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

EBFP2-P2A-Blasticidin S Deaminase

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1alpha

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pJM454 EF1alpha EBFP2-P2A-BlastR (TUPV5)

Plasmid

#139234

Purpose

mMoClo TUPV5, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

EBFP2-P2A-Blasticidin S Deaminase

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1alpha

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pJM453 EF1alpha EBFP2-P2A-BlastR (TUPV4)

Plasmid

#139233

Purpose

mMoClo TUPV4, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

EBFP2-P2A-Blasticidin S Deaminase

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1alpha

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pJM452 EF1alpha EBFP2-P2A-BlastR (TUPV3)

Plasmid

#139232

Purpose

mMoClo TUPV3, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

EBFP2-P2A-Blasticidin S Deaminase

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1alpha

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pJM451 EF1alpha EBFP2-P2A-BlastR (TUPV2)

Plasmid

#139231

Purpose

mMoClo TUPV2, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

EBFP2-P2A-Blasticidin S Deaminase

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1alpha

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pPD882 FKBP-ZF43 (NES)

Plasmid

#138854

Purpose

Constitutive expression of FKBP-ZF43 (NES) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

FKBP-ZF43 (NES)

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPD881 VPR-FRB (NES)

Plasmid

#138853

Purpose

Constitutive expression of VPR-FRB (NES) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VPR-FRB (NES)

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPD880 VP64-FRB (NES)

Plasmid

#138852

Purpose

Constitutive expression of VP64-FRB (NES) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP64-FRB (NES)

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPD684 VPR-FRB (no NLS)

Plasmid

#138851

Purpose

Constitutive expression of VPR-FRB (no NLS) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VPR-FRB (no NLS)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPD683 VP64-FRB (no NLS)

Plasmid

#138850

Purpose

Constitutive expression of VP64-FRB (no NLS) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP64-FRB (no NLS)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pPD682 VP16-FRB (no NLS)

Plasmid

#138849

Purpose

Constitutive expression of VP16-FRB (no NLS) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-FRB (no NLS)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pPD681 FKBP-ZF43 (no NLS)

Plasmid

#138848

Purpose

Constitutive expression of FKBP-ZF43 (no NLS) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

FKBP-ZF43 (no NLS)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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