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We narrowed to 441 results for: T7 rna polymerase

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Showing: 361 - 380 of 441 results

  2. pET28a(+)::NL3F10H

    Plasmid
    #141290
    Purpose
    NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)
    Depositor
    Andrew Millar
    Publication
    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10
    Insert
    NLUC3F10H
    Use
    Luciferase
    Tags
    Flag (x3) and His (x10)
    Expression
    Bacterial
    Mutation
    Engineered for high stability (t1/2 = 11.5 days a…
    Promoter
    promoter for bacteriophage T7 RNA polymerase
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    141290-plasmid-map-sequence-id-324909

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  3. pDB007ns2a

    Plasmid
    #99214
    Purpose
    Accessory plasmid for PACE of PylRS variants; negative selection
    Depositor
    David Liu
    Publication
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); Ptet [SD4] T7RNAP(S12*,S203*)
    Mutation
    amber codons at positions 12 and 203 of T7 RNA po…
    Promoter
    Ppsp, PproK, and Ptet
    Available since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    99214-plasmid-map-sequence-id-192369

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  4. pDB023f1

    Plasmid
    #99218
    Purpose
    Complementary plasmid for aaRS PACE; amber codons in T7 RNAP
    Depositor
    David Liu
    Publication
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD4] T7RNAP(S12*,S203*)
    Expression
    Bacterial
    Mutation
    amber codons at positions 12 and 203 of T7 RNA po…
    Promoter
    Ppsp
    Available since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99218-plasmid-map-sequence-id-192402

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  7. AV15_pCAG.Cas9.gRNA.S1

    Plasmid
    #199259
    Purpose
    Expression construct encoding SpCas9 nuclease and an AAVS1-targeting guide RNA
    Depositor
    Manuel Goncalves
    Publication
    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    SpCas9 nuclease
    AAVS1-targeting gRNA
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG promoter and RNA polymerase III promoter for …
    Available since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199259-plasmid-map-sequence-id-391662

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  8. AV44_pCAG.Cas9-D10A.gRNA.S1

    Plasmid
    #199258
    Purpose
    Expression construct encoding SpCas9-D10A nickase and an AAVS1-targeting guide RNA
    Depositor
    Manuel Goncalves
    Publication
    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    SpCas9-D10A nickase
    AAVS1-targeting gRNA
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    CAG promoter and RNA polymerase III promoter for …
    Available since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199258-plasmid-map-sequence-id-391654

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  10. pMuLE ENTR 7SK L1-L4

    Plasmid
    #62110
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter for shRNA expression. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Publication
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Type
    Empty backbone
    Use
    Gateway Cloning and RNAi
    Expression
    Mammalian
    Available since
    Jan. 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    62110-plasmid-map-sequence-id-101399

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  11. pDB036d

    Plasmid
    #99216
    Purpose
    Accessory plasmid for PACE of MjTyrRS variants; negative selection
    Depositor
    David Liu
    Publication
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); PproA [SD4] T7RNAP(S12*,S203*)
    Expression
    Bacterial
    Mutation
    amber codons at positions 12 and 203 of T7 RNA po…
    Promoter
    Ppsp, PproK, and PproA
    Available since
    Nov. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    99216-plasmid-map-sequence-id-198818

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  12. AV13_pCAG.Cas9.gRNA.NT

    Plasmid
    #199260
    Purpose
    Expression construct encoding SpCas9 nuclease and a control non-targeting guide RNA
    Depositor
    Manuel Goncalves
    Publication
    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    SpCas9 nuclease
    Non-targeting (NT) control gRNA
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG promoter and RNA polymerase III promoter for …
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    199260-plasmid-map-sequence-id-391374

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  13. pET28a(+)::MBP-NL3F10H

    Plasmid
    #141291
    Purpose
    MBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)
    Depositor
    Andrew Millar
    Publication
    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10
    Insert
    MBP-NLUC3F10H
    Use
    Luciferase
    Tags
    Flag (x3), His (x10), and MBP
    Expression
    Bacterial
    Mutation
    Engineered for high stability (t1/2 = 11.5 days a…
    Promoter
    promoter for bacteriophage T7 RNA polymerase
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    141291-plasmid-map-sequence-id-324616

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  15. pDB036a

    Plasmid
    #99215
    Purpose
    Accessory plasmid for PACE of MjTyrRS variants; negative selection
    Depositor
    David Liu
    Publication
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); PproD [SD4] T7RNAP(S12*,S203*)
    Expression
    Bacterial
    Mutation
    amber codons at positions 12 and 203 of T7 RNA po…
    Promoter
    Ppsp, PproK and PproD
    Available since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99215-plasmid-map-sequence-id-192376

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  16. pDB023k

    Plasmid
    #99219
    Purpose
    Complementary plasmid for aaRS PACE; amber codons in T7 RNAP
    Depositor
    David Liu
    Publication
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ppsp [SD8] T7RNAP(S12*,S203*,S527*)
    Mutation
    amber codons at positions 12, 203, and 527 of T7 …
    Promoter
    Ppsp
    Available since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99219-plasmid-map-sequence-id-192936

    Enlarge

  17. pMuLE ENTR 7SK R4-R3

    Plasmid
    #62109
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter for shRNA expression. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Publication
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Type
    Empty backbone
    Use
    Gateway Cloning and RNAi
    Expression
    Mammalian
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62109-plasmid-map-sequence-id-101398

    Enlarge

  18. pMuLE ENTR 7SK L5-L2

    Plasmid
    #62108
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter for shRNA expression. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Publication
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Type
    Empty backbone
    Use
    Gateway Cloning and RNAi
    Expression
    Mammalian
    Available since
    Feb. 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    62108-plasmid-map-sequence-id-101397

    Enlarge

  19. pMuLE ENTR 7SK L5-L4

    Plasmid
    #62107
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter for shRNA expression. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Publication
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Type
    Empty backbone
    Use
    Gateway Cloning and RNAi
    Expression
    Mammalian
    Available since
    Feb. 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    62107-plasmid-map-sequence-id-101396

    Enlarge

  20. pMuLE ENTR 7SK L1-R5

    Plasmid
    #62106
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter for shRNA expression. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Publication
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Type
    Empty backbone
    Use
    Gateway Cloning and RNAi
    Expression
    Mammalian
    Available since
    Feb. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62106-plasmid-map-sequence-id-101395

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Showing: 361 - 380 of 441 results