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(Plasmid #99218)


Item Catalog # Description Quantity Price (USD)
Plasmid 99218 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    David Liu Lab
  • Backbone size w/o insert (bp) 2729
  • Total vector size (bp) 5620
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Ppsp [SD4] T7RNAP(S12*,S203*)
  • Species
  • Insert Size (bp)
  • Mutation
    amber codons at positions 12 and 203 of T7 RNA polymerase
  • Promoter Ppsp

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDB023f1 was a gift from David Liu (Addgene plasmid # 99218 ; ; RRID:Addgene_99218)
  • For your References section:

    Continuous directed evolution of aminoacyl-tRNA synthetases. Bryson DI, Fan C, Guo LT, Miller C, Soll D, Liu DR. Nat Chem Biol. 2017 Oct 16. doi: 10.1038/nchembio.2474. 10.1038/nchembio.2474 PubMed 29035361
Commonly requested with: