We narrowed to 15,621 results for: pET

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mouse KANK4 Ankyrin Repeats pET151

Plasmid

#164057

Purpose

Expresses mouse KANK4 Ank domain in bacterial cells

Depositor

Ben Goult

Insert

Kidney Ankyrin Repeat-Containing Protein 4 (Kank4 Mouse)

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pET-Duet1-6xHis-TEV-NDP52

Plasmid

#190194

Purpose

Plasmid for the protein expression of 6xHis-TEV-NDP52 in a bacterial expression system. Internal reference: SMC401

Depositor

Sascha Martens

Article

Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

NDP52 (CALCOCO2 Human)

Tags

6xHis-TEV

Expression

Bacterial

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pET_His6-MBP-TEV-Cas6-RT-Cas1_WT

Plasmid

#186274

Purpose

Expresses Thiomicrospira Cas6-RT-Cas1 in E. coli

Depositor

Jennifer Doudna

Article

Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

Insert

Thiomicropira Cas6-RT-Cas1

Tags

His6-MBP

Expression

Bacterial

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pET-Duet1-6xHis-TEV-OPTN

Plasmid

#190192

Purpose

Plasmid for the expression of 6xHis-TEV-OPTN. Internal reference: SMC397

Depositor

Sascha Martens

Article

Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

optineurin (OPTN Human)

Tags

6xHis and TEV

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pET-His6-Sumo-CylK-Strep

Plasmid

#185962

Purpose

Expresses His6-Sumo-CylK-Strep in E. coli for refolding protein preparation

Depositor

Emily Balskus

Article

Braffman et al Elife. 2022 Feb 25;11. pii: 75761. doi:

Insert

CylK

Tags

His6-Sumo

Expression

Bacterial

Promoter

T7

Available Since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pETDuet1-LanCL2-H264A-C-Ter-His

Plasmid

#154187

Purpose

To express LanCL2-H264A in bacterial cells

Depositor

Wilfred van der Donk

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

LanCL2

Tags

Hexahistidine

Expression

Bacterial

Mutation

His264->Ala264

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET Duet1 His6 SUMO D14

Plasmid

#177854

Purpose

Bacterial Expression of D14

Depositor

Karsten Melcher

Article

Zhao et al Cell Res. 2013 Mar;23(3):436-9. doi: 10.

Insert

D14

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-Fgβ-anticalin-His-ybbr

Plasmid

#168040

Purpose

Expresses anticalin targeting CTLA-4 with Fgβ peptide at the N terminus and 6x-His tag and ybbr tag at the C terminus

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag, Fgβ peptide, and ybbr tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET15b p97-Strep-His E314Amb

Plasmid

#169021

Purpose

Expresses p97-Strep-His E314Amb (incorporates noncanonical amino acid at position 314 via amber suppression) in bacteria

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p97

Tags

His and SBP

Expression

Bacterial

Mutation

E314TAG

Promoter

T7

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-SNAP-tag_dead_mt

Plasmid

#167270

Purpose

Overexpression of the dead variant (C145A) of SNAP-tag in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

SNAP-tag

Tags

His x10

Expression

Bacterial

Mutation

C145A

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-SNAP-tag_fast_dead_mt

Plasmid

#167272

Purpose

Overexpression of the fast (E30R) dead variant (C145A) of SNAP-tag in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

SNAPf-tag

Tags

His x10

Expression

Bacterial

Mutation

E30R-C145A

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3567 (pET151_Spt6 1247-1451 R1282H)

Plasmid

#91816

Purpose

Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with an R1282H mutation

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

SPT6 (SPT6 Budding Yeast)

Tags

6xHis

Expression

Bacterial

Mutation

changed arginine 1282 to histidine; deleted resid…

Promoter

T7

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3495 (pET151_Spt6 1247-1451 K1435A)

Plasmid

#91817

Purpose

Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with a K1435A mutation

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

SPT6 (SPT6 Budding Yeast)

Tags

6xHis

Expression

Bacterial

Mutation

changed lysine 1435 to alanine; deleted residues …

Promoter

T7

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pET-11a (GB1 WT - A34F/V39L)

Plasmid

#136610

Purpose

Expresses GB1 WT double mutant (A34F/V39L)

Depositor

Roberto Chica

Article

Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

Insert

GB1 WT double mutant (A34F/V39L)

Tags

6x-Histag

Expression

Bacterial

Mutation

Mutation from GB1 WT: A34F - V39L

Promoter

T7 promoter

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-Ypet-3XNLS-WPRE

Plasmid

#159950

Purpose

nucleus marker

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

Ypet-3XNLS

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-Ypet-optoJNKi-WPRE

Plasmid

#159967

Purpose

optogenetic JNK inhibitor with increased RET from LOV2 to reduce actuation sensitivity, detectable without activation

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

Ypet-optoJNKi

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pET-45b-HOXA13(+7A)-IDR-mCherry

Plasmid

#145285

Purpose

Bacterial Expression of 6xHis HOXA13(+7A)IDR mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXA13(+7A)-IDR (HOXA13 Human)

Expression

Bacterial

Mutation

A-repeat expanded

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pET-45b-HOXA13(WT)-IDR-mCherry

Plasmid

#145284

Purpose

Bacterial Expression of 6xHis HOXA13(WT) IDR mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXA13(WT)-IDR (HOXA13 Human)

Expression

Bacterial

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pET15-His-∆NGlpG(C104A/M247C)

Plasmid

#155133

Purpose

Expresses N-terminally His-tagged E. coli GlpG C104A/M247C variant (residues 87-276) from pET15b

Depositor

Sinisa Urban

Article

Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

Insert

GlpG (glpG Escherichia coli)

Tags

His

Expression

Bacterial

Mutation

C104A/M247C, insert codes for GlpG residues 87-276

Promoter

T7

Available Since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-Avi-Coh (E154C)-HIS

Plasmid

#153447

Purpose

E. coli expression of Rc. CohE construct with E154C mutation.

Depositor

Michael Nash

Article

Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

Insert

Rc.CohE

Tags

6xHis and Avi-tag

Expression

Bacterial

Mutation

E154C mutation on CohE

Promoter

T7 promoter

Available Since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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