168,579 results

1. pAAV-hSYN1-fDIO-tTA

   Plasmid

   #166597

   Purpose

   Encodes Flp-dependent tTA

   Depositor

   Martin Myers

   Article

   Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

   Insert

   tTA
   

   Use

   AAV

   Promoter

   human synapsin 1

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV.TBG.PI.eGFP.WPRE.bGH

   Plasmid

   #105535

   Purpose

   AAV expression of EGFP from TBG promoter

   Depositor

   James M. Wilson

   Has Service

   AAV8

   Insert

   EGFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   TBG

   Available Since

   Feb. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. HMA-APOBEC1-Nb

   Plasmid

   #226012

   Purpose

   For expression of His-MBP-APOBEC1-Nanobody protein in E.coli

   Depositor

   Eugene Yeo

   Article

   Liang et al Nat Commun. 2024 Aug 16;15(1):7067. doi:

   Insert

   APOBEC1 (Apobec1 Rat)
   

   Tags

   His-MBP and antiRabbit-nanobody

   Expression

   Bacterial

   Available Since

   Oct. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-C1 EGFP-3XNLS

   Plasmid

   #58468

   Purpose

   Expresses a nuclear-targeted eGFP on a CMV promoter

   Depositor

   Dyche Mullins

   Article

   Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

   Insert

   3X NLS with 3X stop codons
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. LcV2-Hygro

   Plasmid

   #91977

   Purpose

   LentiCRISPR v2 with Hygro cloned in place of Puro

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-N1 Dynamin1 wt

   Plasmid

   #120313

   Purpose

   Mammalian expression plasmid of GFP-tagged Dynamin protein.

   Depositor

   Justin Taraska

   Article

   Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

   Insert

   Dynamin1 (DNM1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CAG-mNeonGreen

   Plasmid

   #99134

   Purpose

   An AAV genome that ubiquitously expresses the fluorescent protein mNeonGreen from the CAG promoter

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Insert

   mNeonGreen
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pC1-CMV-mito-DAAO

   Plasmid

   #141132

   Purpose

   Expresses DAAO with mitochondrial localization signal in mammalian cells

   Depositor

   Vsevolod Belousov

   Insert

   mito-DAAO
   

   Tags

   2 mitochondrial localization signals and 7xHis

   Expression

   Mammalian

   Mutation

   deleted DAAO amino acids 367-368

   Promoter

   CMV

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZR158_Lenti-U6-gRNA-10xCS1-PP7_hPGK-PCP-P65-HSF1-Puro

   Plasmid

   #180273

   Purpose

   Lentiviral expression vector for CRISPRa-SAM sgRNA with 10x capture sequence, and PCP-P65-HSF1 cassette

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-Anillin

    Plasmid

    #68027

    Purpose

    Full length Anillin rendered RNAi-resistant) in mammalian expression vector pEGFP-C1

    Depositor

    Michael Glotzer

    Article

    Piekny et al Curr Biol. 2008 Jan 8;18(1):30-6. Epub 2

    Insert

    Anillin (ANLN Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    silent mutations at 798 5'-TGCC TCTTTGAATAAA…

    Promoter

    CMV

    Available Since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO-Tet-On-shRNA-Control

    Plasmid

    #98398

    Purpose

    Lentivirus for expression of non-targeting control shRNA (Dox-inducible)

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    non-targeting randomized sequence
    

    Use

    Lentiviral

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFH3.3_Cre-MS2x12

    Plasmid

    #205559

    Purpose

    Express Cre-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    Cre
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

    Plasmid

    #139987

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. Mouse Metabolism Library

    Pooled Library

    #163966

    Purpose

    Lentiviral CRISPR library targets 2,918 mouse metabolic genes with ~18,000 guide RNAs. The library contains ~6 gRNAs per gene.

    Depositor

    Alec Kimmelman

    Article

    Biancur et al Cell Metab. 2020 Oct 28. pii: S1550-4131

    Use

    CRISPR and Lentiviral

    Available Since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3-Flag-IDH1-R132H

    Plasmid

    #62907

    Purpose

    Expresses R132 mutant IDH1 in mammalian cells

    Depositor

    Yue Xiong

    Article

    Zhao et al Science. 2009 Apr 10;324(5924):261-5. do

    Insert

    IDH1 (IDH1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deleted Arg132 (R132)

    Available Since

    April 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMT416

    Plasmid

    #8607

    Depositor

    Robert Hartley

    Article

    Hartley et al J Mol Biol 1988 Aug 20;202(4):913-5.

    Insert

    barnase, barstar
    

    Tags

    phoA signal sequence to barnase

    Expression

    Bacterial

    Available Since

    Jan. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21a-MTF-His

    Plasmid

    #124133

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Methionyl-tRNA formyltransferase (fmt Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. C321.∆A T7RNAP ompT- rne- lon-

    Bacterial Strain

    #182778

    Purpose

    Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT, rne, and lon inactivated

    Depositor

    Michael Jewett

    Article

    Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

    Bacterial Resistance

    Spectinomycin

    Available Since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

19. zCas9i cloning kit: MoClo-compatible CRISPR/Cas9 cloning kit with intronized Cas9

    Plasmid Kit

    #1000000171

    Purpose

    Vectors and DNA modules for assembly of CRISPR/Cas9 constructs for use in plants. The constructs are made with zCas9i, a high efficiency intron-optimized SpCas9-coding gene.

    Depositor

    Sylvestre Marillonnet, Johannes Stuttmann

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

20. FUW-Luc-mCherry-puro

    Plasmid

    #183685

    Purpose

    Lentiviral vector expressing luciferase and mCherry.

    Depositor

    Andrew Kung

    Article

    Kimbrel et al Mol Imaging. 2009 May-Jun;8(3):140-7.

    Insert

    Luc-E2A-mCherry-T2A-PuroR
    

    Use

    Lentiviral

    Promoter

    Human Ubiquitin C (UbC) promoter

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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